Kinesin is an evolutionarily fine-tuned molecular ratchet-and-pawl device of decisively locked direction
文献类型：期刊论文
| 作者 | Wang, Zhisong; Feng, Min; Zheng, Wenwei; Fan, Dagong |
| 刊名 | BIOPHYSICAL JOURNAL
 |
| 出版日期 | 2007-11-01 |
| 卷号 | 93页码:3363-3372 |
| ISSN号 | 0006-3495 |
| DOI | 10.1529/biophysj.107.108233 |
| 英文摘要 | Conventional kinesin is a dimeric motor protein that transports membranous organelles toward the plus-end of microtubules (MTs). Individual kinesin dimers show steadfast directionality and hundreds of consecutive steps, yet the detailed physical mechanism remains unclear. Here we compute free energies for the entire dimer-MT system for all possible interacting configurations by taking full account of molecular details. Employing merely first principles and several measured binding and barrier energies, the system-level analysis reveals insurmountable energy gaps between configurations, asymmetric ground state caused by mechanically lifted configurational degeneracy, and forbidden transitions ensuring coordination between both motor domains for alternating catalysis. This wealth of physical effects converts a kinesin dimer into a molecular ratchet-and-pawl device, which determinedly locks the dimer's movement into the MT plus-end and ensures consecutive steps in hand-over-hand gait. Under a certain range of extreme loads, however, the ratchet- and-pawl device becomes defective but not entirely abolished to allow consecutive back-steps. This study yielded quantitative evidence that kinesin's multiple molecular properties have been evolutionarily adapted to fine-tune the ratchet-and-pawl device so as to ensure the motor's distinguished performance. |
| WOS关键词 | HEREDITARY SPASTIC PARAPLEGIA ; HAND-OVER-HAND ; ATP HYDROLYSIS ; ENERGY-TRANSFER ; BROWNIAN MOTOR ; CATALYTIC CORE ; PROCESSIVITY ; MECHANISM ; HEAD ; MOTILITY |
| WOS研究方向 | Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000250577700004 |
| 出版者 | BIOPHYSICAL SOC |
| 源URL | [http://119.78.100.186/handle/113462/27139]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Wang, Zhisong |
| 作者单位 | 1.Fudan Univ, Inst Modern Phys, Appl Ion Beam Phys, Shanghai, Peoples R China 2.Univ Sussex, Genome Damage & Stabil Ctr, Brighton BN1 9RH, E Sussex, England |
| 推荐引用方式 GB/T 7714 | Wang, Zhisong,Feng, Min,Zheng, Wenwei,et al. Kinesin is an evolutionarily fine-tuned molecular ratchet-and-pawl device of decisively locked direction[J]. BIOPHYSICAL JOURNAL,2007,93:3363-3372. |
| APA | Wang, Zhisong,Feng, Min,Zheng, Wenwei,&Fan, Dagong.(2007).Kinesin is an evolutionarily fine-tuned molecular ratchet-and-pawl device of decisively locked direction.BIOPHYSICAL JOURNAL,93,3363-3372. |
| MLA | Wang, Zhisong,et al."Kinesin is an evolutionarily fine-tuned molecular ratchet-and-pawl device of decisively locked direction".BIOPHYSICAL JOURNAL 93(2007):3363-3372. |
入库方式： OAI收割
来源：近代物理研究所
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