A native Zn/Cd pumping P-1B ATPase from natural overexpression in a hyperaccumulator plant
文献类型：期刊论文
| 作者 | Parameswaran, Aravind; Leitenmaier, Barbara; Yang, Mingjie; Kroneck, Peter M. H.; Welte, Wolfram; Lutz, Gabriela; Papoyan, Ashot; Kochian, Leon V.; Kuepper, Hendrik |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2007-11-09 |
| 卷号 | 363页码:51-56 |
| 关键词 | cadmium heavy metals metal activation P-1B type ATPase TcHMA4 zinc |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbre.2007.08.105 |
| 英文摘要 | We report here the first purification of a P-1B type ATPase, a group of transporters that occurs in bacteria, plants and animals incl. humans, from a eukaryotic organism in native state. TcHMA4 is a P-1B type ATPase that is highly expressed in the Cd/Zn hyperaccumulator plant Thlaspi caerulescens and contains a C-terminal 9-histidine repeat. After isolation from roots, we purified TcHMA4 protein via metal affinity chromatography. The purified protein exhibited Cd- and Zn-activated ATPase activity after reconstitution into lipid vesicles, showing that it was in its native state. Gels of crude root extract and of the purified protein revealed TcHMA4-specific bands of about 50 and 60 kDa, respectively, while the TcHMA4 mRNA predicts a single protein with a size of 128 kDa. This indicates the occurrence of post-translational processing; the properties of the two bands were characterised by their activity and binding properties. (C) 2007 Elsevier Inc. All rights reserved. |
| WOS关键词 | HEAVY-METAL TRANSPORT ; P-TYPE ATPASES ; THLASPI-CAERULESCENS ; ARABIDOPSIS-HALLERI ; GENES ; PHYTOREMEDIATION ; ENVIRONMENT ; EXPRESSION ; ZINC |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000250060200009 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 源URL | [http://119.78.100.186/handle/113462/27381]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Kuepper, Hendrik |
| 作者单位 | 1.Univ Konstanz, Fachbereich Biol, D-78457 Constance, Germany 2.Fudan Univ, Inst Modern Phys, Shanghai 200433, Peoples R China 3.Cornell Univ, US Plant Soil & Nutr Lab, USDA ARS, Ithaca, NY 14853 USA 4.Univ S Bohemia, Fac Biol Sci, CZ-37005 Ceske Budejovice, Czech Republic 5.Univ S Bohemia, Inst Phys Biol, CZ-37005 Ceske Budejovice, Czech Republic |
| 推荐引用方式 GB/T 7714 | Parameswaran, Aravind,Leitenmaier, Barbara,Yang, Mingjie,et al. A native Zn/Cd pumping P-1B ATPase from natural overexpression in a hyperaccumulator plant[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2007,363:51-56. |
| APA | Parameswaran, Aravind.,Leitenmaier, Barbara.,Yang, Mingjie.,Kroneck, Peter M. H..,Welte, Wolfram.,...&Kuepper, Hendrik.(2007).A native Zn/Cd pumping P-1B ATPase from natural overexpression in a hyperaccumulator plant.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,363,51-56. |
| MLA | Parameswaran, Aravind,et al."A native Zn/Cd pumping P-1B ATPase from natural overexpression in a hyperaccumulator plant".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 363(2007):51-56. |
入库方式： OAI收割
来源：近代物理研究所
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