Symmetry-adapted spherical harmonics method for high-resolution 3D single-particle reconstructions
文献类型：期刊论文
| 作者 | Liu, Hongrong1; Cheng, Lingpeng2,3; Zeng, Songjun1; Cai, Canying1; Zhou, Z. Hong2,3; Yang, Qibin1 |
| 刊名 | JOURNAL OF STRUCTURAL BIOLOGY
 |
| 出版日期 | 2008 |
| 卷号 | 161页码:64-73 |
| 关键词 | cryo-electron microscopy virus structure three-dimensional reconstruction icosahedral symmetry-adapted functions |
| ISSN号 | 1047-8477 |
| DOI | 10.1016/j.jsb.2007.09.016 |
| 英文摘要 | Three-dimensional (3D) reconstruction is the last and an essential step toward high-resolution structural determination in single-particle cryo-electron microscopy (cryoEM). We have implemented a new algorithm for reconstructing 3D structures of macromolecular complexes with icosahedral symmetry from cryoEM images. Icosahedral symmetry-adapted functions (ISAFs) are used to interpolate structural factors in the reciprocal space to generate a 3D reconstruction in spherical coordinates. In our implementation, we introduced a recursive method for deriving higher order ISAFs from three lower order seed functions. We demonstrate improvements of our new method in both the noise suppression and the effective resolution in 3D reconstruction over the commonly used Fourier-Bessel synthesis method introduced by Crowther et al. three decades ago. Our 3D reconstruction method can be extended to macromolecular complexes with other symmetry types and is thus likely to impact future high-resolution cryoEM single-particle reconstruction efforts in general. (c) 2007 Elsevier Inc. All rights reserved. |
| WOS关键词 | ELECTRON CRYOMICROSCOPY ; 3-DIMENSIONAL RECONSTRUCTION ; CRYOELECTRON MICROSCOPY ; ICOSAHEDRAL GROUP ; ANGSTROM RESOLUTION ; DISORDERED SPECIMENS ; VIRUS STRUCTURES ; MICROGRAPHS ; PROTEIN ; MODEL |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000252228200007 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 源URL | [http://119.78.100.186/handle/113462/28250]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Yang, Qibin |
| 作者单位 | 1.Xiangtan Univ, Inst Modern Phys, Xiangtan 411105, Hunan, Peoples R China 2.Univ Calif Los Angeles, Dept Microbiol Mol Genet & Immunol, Los Angeles, CA 90095 USA 3.Univ Calif Los Angeles, Calif Nanosyst Inst, Los Angeles, CA 90095 USA |
| 推荐引用方式 GB/T 7714 | Liu, Hongrong,Cheng, Lingpeng,Zeng, Songjun,et al. Symmetry-adapted spherical harmonics method for high-resolution 3D single-particle reconstructions[J]. JOURNAL OF STRUCTURAL BIOLOGY,2008,161:64-73. |
| APA | Liu, Hongrong,Cheng, Lingpeng,Zeng, Songjun,Cai, Canying,Zhou, Z. Hong,&Yang, Qibin.(2008).Symmetry-adapted spherical harmonics method for high-resolution 3D single-particle reconstructions.JOURNAL OF STRUCTURAL BIOLOGY,161,64-73. |
| MLA | Liu, Hongrong,et al."Symmetry-adapted spherical harmonics method for high-resolution 3D single-particle reconstructions".JOURNAL OF STRUCTURAL BIOLOGY 161(2008):64-73. |
入库方式： OAI收割
来源：近代物理研究所
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