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The intrinsic load-resisting capacity of kinesin

文献类型：期刊论文

作者Zheng, Wenwei3; Fan, Dagong3; Feng, Min4; Wang, Zhisong1,2
刊名PHYSICAL BIOLOGY
出版日期2009-09-01
卷号6页码:13
ISSN号1478-3967
DOI10.1088/1478-3975/6/3/036002
英文摘要Conventional kinesin is a homodimeric motor protein that is capable of walking unidirectionally along a cytoskeletal filament. While previous experiments indicated unyielding unidirectionality against an opposing load up to the so-called stall force, recent experiments also observed limited processive backwalking under superstall loads. This theoretical study seeks to elucidate the molecular mechanical basis for kinesin's steps over the full range of external loads that can possibly be applied to the dimer. We found that kinesin's load-resisting capacity is largely determined by a synergic ratchet-and-pawl mechanism inherent in the dimer. Load susceptibility of this inner molecular mechanical mechanism underlies kinesin's response to various levels of external loads. Computational implementation of the mechanism enabled us to rationalize major trends observed experimentally in kinesin's stalemate and consecutive back steps. The study also predicts several distinct features of kinesin's load-affected motility, which are seemingly counterintuitive but readily verifiable by future experiment.
WOS关键词HAND-OVER-HAND ; ATP HYDROLYSIS ; FORCE CLAMP ; MOTOR ; MOLECULES ; MECHANISM ; MOTILITY ; PROTEIN ; STEPS ; HEAD
资助项目NUS
WOS研究方向Biophysics
语种英语
WOS记录号WOS:000269178800002
出版者IOP PUBLISHING LTD
资助机构NUS
源URL[http://119.78.100.186/handle/113462/30403]  
专题中国科学院近代物理研究所
通讯作者Wang, Zhisong
作者单位1.Natl Univ Singapore, Dept Phys, Singapore 117542, Singapore
2.Natl Univ Singapore, Ctr Computat Sci & Engn, Singapore 117542, Singapore
3.Fudan Univ, Inst Modern Phys, Shanghai 200433, Peoples R China
4.Shanghai Canc Inst, Shanghai 200032, Peoples R China
推荐引用方式
GB/T 7714		 Zheng, Wenwei,Fan, Dagong,Feng, Min,et al. The intrinsic load-resisting capacity of kinesin[J]. PHYSICAL BIOLOGY,2009,6:13.
APA Zheng, Wenwei,Fan, Dagong,Feng, Min,&Wang, Zhisong.(2009).The intrinsic load-resisting capacity of kinesin.PHYSICAL BIOLOGY,6,13.
MLA Zheng, Wenwei,et al."The intrinsic load-resisting capacity of kinesin".PHYSICAL BIOLOGY 6(2009):13.

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来源：近代物理研究所

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