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Low-dose carbon ion irradiation effects on DNA damage and oxidative stress in the mouse testis

文献类型：期刊论文

作者Liu, Yang1,2,3,4; Long, Jing1,3,4; Zhang, Luwei1,3,4; Zhang, Hong1,3,4; Liu, Bin1,3,4; Zhao, Weiping1,3,4; Wu, Zhehua1,3,4
刊名ADVANCES IN SPACE RESEARCH
出版日期2011-01-04
卷号47页码:55-59
关键词Carbon ions DNA double-strand breaks (DNA DSBs) Malondialdehyde (MDA) Superoxide dismutase (SOD) Mouse testis
ISSN号0273-1177
DOI10.1016/j.asr.2010.08.037
英文摘要To investigate the effects of low-dose carbon ion irradiation on reproductive system of mice, the testes of outbred Kunming strain mice were whole-body irradiated with 0, 0.05, 0.1, 0.5 and 1 Gy, respectively. We measured DNA double-strand breaks (DNA DSBs) and oxidative stress parameters including malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, and testis weight and sperm count at 12 h, 21 d and 35 d after irradiation in mouse testis. At 12 h postirradiation, a significant increase in DNA DSB level but no pronounced alterations in MDA content or SOD activity were observed in 0.5 and 1 Gy groups compared with the control group. At 21 d postirradiation, there was a significant reduction in sperm count and distinct enhancements of DSB level and MDA content in 0.5 and 1 Gy groups in comparison with control. At 35 d postirradiation, the levels of DNA DSBs and MDA, and SOD activity returned to the baseline except for the MDA content in 1 Gy (P < 0.05), while extreme falls of sperm count were still observed in 0.5 (P < 0.01) and 1 Gy (P < 0.01) groups. For the 0.05 or 0.1 Gy group, no differences were found in DNA DSB level and MDA content between control and at 12 h, 21 d and 35 d after irradiation, indicating that lower doses of carbon ion irradiation have no significant influence on spermatogenesis processes. In this study, male germ cells irradiated with over 0.5 Gy of carbon ions are difficult to repair completely marked by the sperm count. Furthermore, these data suggest that the deleterious effects may be chronic or delayed in reproductive system after whole-body exposure to acute high-dose carbon ions. (c) 2010 COSPAR. Published by Elsevier Ltd. All rights reserved.
WOS关键词DOUBLE-STRAND-BREAKS ; COMET ASSAY ; RADIATION ; REPAIR ; SPERMATOCYTES ; SPERMATOGONIA ; PREEXPOSURE ; CELL ; AGE
资助项目National Basic Research Program of China[2010CB834202] ; National Natural Science Foundation of China[10835011] ; National Natural Science Foundation of China[10675151] ; National Natural Science Foundation of China[10805064] ; Scientific Technology Research Projects of Gansu Province[0702NKDA045] ; Scientific Technology Research Projects of Gansu Province[0806RJYA 020] ; Chinese Academy of Sciences[0760160XBO] ; Chinese Academy of Sciences[0860260XBO]
WOS研究方向Astronomy & Astrophysics ; Geology ; Meteorology & Atmospheric Sciences
语种英语
WOS记录号WOS:000287104500006
出版者ELSEVIER SCI LTD
资助机构National Basic Research Program of China ; National Natural Science Foundation of China ; Scientific Technology Research Projects of Gansu Province ; Chinese Academy of Sciences
源URL[http://119.78.100.186/handle/113462/33942]  
专题中国科学院近代物理研究所
通讯作者Zhang, Hong
作者单位1.Chinese Acad Sci, Inst Modern Phys, Dept Med Phys, Lanzhou 730000, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Key Lab Heavy Ion Radiat Med Gansu Prov, Lanzhou 730000, Peoples R China
4.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Peoples R China
推荐引用方式
GB/T 7714		 Liu, Yang,Long, Jing,Zhang, Luwei,et al. Low-dose carbon ion irradiation effects on DNA damage and oxidative stress in the mouse testis[J]. ADVANCES IN SPACE RESEARCH,2011,47:55-59.
APA Liu, Yang.,Long, Jing.,Zhang, Luwei.,Zhang, Hong.,Liu, Bin.,...&Wu, Zhehua.(2011).Low-dose carbon ion irradiation effects on DNA damage and oxidative stress in the mouse testis.ADVANCES IN SPACE RESEARCH,47,55-59.
MLA Liu, Yang,et al."Low-dose carbon ion irradiation effects on DNA damage and oxidative stress in the mouse testis".ADVANCES IN SPACE RESEARCH 47(2011):55-59.

入库方式： OAI收割

来源：近代物理研究所

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