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Effects of X-irradiation on mitochondrial DNA damage and its supercoiling formation change

文献类型：期刊论文

作者Zhou, Xin1,2,3,4,6; Li, Ning1,2,3,4,6; Wang, Yanling1,2,3,6; Wang, Yipei5; Zhang, Xin1,2,3,6; Zhang, Hong1,2,3,6
刊名MITOCHONDRION
出版日期2011-11-01
卷号11页码:886-892
关键词Mitochondrial DNA Irradiation Supercoiling formation Long PCR
ISSN号1567-7249
DOI10.1016/j.mito.2011.07.005
英文摘要There have been a small number of reports of radiation-induced mtDNA damage, and mtDNA supercoiling formation change induced by ionizing radiation has not been investigated before. This study evaluated mtDNA damage and supercoiling formation change after X-irradiation. The human breast cancer cell line, MCF-7 cells were used for analysis. Modified supercoiling-sensitive real-time PCR approach was used to evaluate mitochondrial DNA supercoiling formation change and copy number; long-PCR method was applied for the quantification of mtDNA damage. MtDNA damage and formation change induced by high-dose irradiation was persistent in 24 h after irradiation and was not significant after low-dose irradiation. MtDNA copy number was slightly increased after high-dose irradiation and a transit increase was observed after low-dose irradiation. This is the first study to evaluate radiation-induced mitochondrial DNA supercoiling formation change using real-time PCR. Combined with data of ROS generation and dynamics of mitochondrial mass, our findings suggested that mtDNA is sensitive to radiation hazards, indicating mitochondrial biogenesis play an important role in radiation-induced cellular response. (C) 2011 Elsevier B.V. and Mitochondria Research Society. All rights reserved.
WOS关键词TUMOR-SUPPRESSOR P53 ; DOUBLE-STRAND BREAKS ; INDUCED CELL-DEATH ; REAL-TIME PCR ; OXIDATIVE STRESS ; IONIZING-RADIATION ; GAMMA-RADIATION ; HUMAN SKIN ; TRANSCRIPTION FACTOR ; SUNLIGHT EXPOSURE
资助项目National Basic Research Program of China (973 Program)[2010CB834202] ; National Natural Science Foundation of China[10835011] ; National Natural Science Foundation of China[10675151] ; National Natural Science Foundation of China[10805064] ; Scientific Technology Research Projects of Gansu Province[0702NKDA045] ; Scientific Technology Research Projects of Gansu Province[0806RJYA020] ; Chinese Academy of Sciences[0760160XBO] ; Chinese Academy of Sciences[0860260XBO]
WOS研究方向Cell Biology ; Genetics & Heredity
语种英语
WOS记录号WOS:000296941300008
出版者ELSEVIER SCI LTD
资助机构National Basic Research Program of China (973 Program) ; National Natural Science Foundation of China ; Scientific Technology Research Projects of Gansu Province ; Chinese Academy of Sciences
源URL[http://119.78.100.186/handle/113462/34465]  
专题中国科学院近代物理研究所
通讯作者Zhang, Hong
作者单位1.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
2.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol, Lanzhou 730000, Peoples R China
3.Chinese Acad Sci, Med Inst Nucl Phys, Lanzhou 730000, Peoples R China
4.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
5.Univ Delaware, Dept Chem & Biochem, Newark, DE 19711 USA
6.Key Lab Heavy Ion Radiat Med Gansu Prov, Lanzhou 730000, Peoples R China
推荐引用方式
GB/T 7714		 Zhou, Xin,Li, Ning,Wang, Yanling,et al. Effects of X-irradiation on mitochondrial DNA damage and its supercoiling formation change[J]. MITOCHONDRION,2011,11:886-892.
APA Zhou, Xin,Li, Ning,Wang, Yanling,Wang, Yipei,Zhang, Xin,&Zhang, Hong.(2011).Effects of X-irradiation on mitochondrial DNA damage and its supercoiling formation change.MITOCHONDRION,11,886-892.
MLA Zhou, Xin,et al."Effects of X-irradiation on mitochondrial DNA damage and its supercoiling formation change".MITOCHONDRION 11(2011):886-892.

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来源：近代物理研究所

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