BRCA1 involved in regulation of Bcl-2 expression and apoptosis susceptibility to ionizing radiation
文献类型：期刊论文
| 作者 | Wang YanLing1,2,3 ; Wang Bing5; Zhang Hong1,2,3; Li Ning1,2,3,4; Tanaka, Kaoru5; Zhou Xin1,2,3,4; Chen RuPing6; Zhang Xin1,2,3,4
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| 刊名 | SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY
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| 出版日期 | 2011-05-01 |
| 卷号 | 54页码:916-922 |
| 关键词 | BRCA1 p53 Bax Bcl-2 apoptosis ionizing radiation |
| ISSN号 | 1674-7348 |
| DOI | 10.1007/s11433-011-4264-6 |
| 英文摘要 | BRCA1 has been proposed to be tightly linked to the resistance of tumor cells to ionizing radiation. The pathway leading to this phenomenon is not yet clear. In this work, we investigated the role of BRCA1 in the apoptosis regulation in response to carbon ion irradiation. We utilized three different cancer cell lines with various states for BRCA1 and p53 to identify the relationship between endogenous BRCA1 and the apoptosis-related genes, and determine whether p53 function would affect the role of BRCA1 in apoptosis regulation. By Western blot analysis, we found that Bax expressions were not significantly changed after irradiation in all of three cell lines. However, Bcl-2 expression showed an up-regulation by endogenous BRCA1 regardless of p53 status. Moreover, the changes in Bcl-2 protein were due to the increase in the transcriptional levels of Bcl-2 mRNA, based on real-time PCR assay. At the same time, BRCA1-deficient cells showed a greater apoptosis susceptibility to irradiation when compared with BRCA1-proficient cells. The results suggest that BRCA1 might exert p53-independent regulative activities for Bcl-2, which seems account for the low apoptosis susceptibility in BRCA1-proficient carcinomas. |
| WOS关键词 | DNA-DAMAGE RESPONSE ; CELL-CYCLE ; TRANSCRIPTIONAL REGULATION ; P53-DEPENDENT APOPTOSIS ; TUMOR-SUPPRESSOR ; BREAST ; P53 ; PROTEINS ; REPAIR ; DEATH |
| WOS研究方向 | Physics |
| 语种 | 英语 |
| WOS记录号 | WOS:000290768300021 |
| 出版者 | SCIENCE PRESS |
| 源URL | [http://119.78.100.186/handle/113462/34476]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Zhang Hong |
| 作者单位 | 1.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Peoples R China 3.Key Lab Heavy Ion Radiat Med Gansu Prov, Lanzhou 730000, Peoples R China 4.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 5.Natl Inst Radiol Sci, Radiat Hazards Res Grp, Chiba, Japan 6.Lanzhou Univ, Coll Pharmaceut Sci, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang YanLing,Wang Bing,Zhang Hong,et al. BRCA1 involved in regulation of Bcl-2 expression and apoptosis susceptibility to ionizing radiation[J]. SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY,2011,54:916-922. |
| APA | Wang YanLing.,Wang Bing.,Zhang Hong.,Li Ning.,Tanaka, Kaoru.,...&Zhang Xin.(2011).BRCA1 involved in regulation of Bcl-2 expression and apoptosis susceptibility to ionizing radiation.SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY,54,916-922. |
| MLA | Wang YanLing,et al."BRCA1 involved in regulation of Bcl-2 expression and apoptosis susceptibility to ionizing radiation".SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY 54(2011):916-922. |
入库方式： OAI收割
来源：近代物理研究所
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