Irradiation Response of Adipose-derived Stem Cells under Three-dimensional Culture Condition
文献类型：期刊论文
| 作者 | Du Ya Rong1; Pan Dong1,3; Chen Ya Xiong1; Xue Gang1,3; Ren Zhen Xin1; Li Xiao Man1,3; Zhang Shi Chuan2; Hu Bu Rong1 |
| 刊名 | BIOMEDICAL AND ENVIRONMENTAL SCIENCES
 |
| 出版日期 | 2015-08-01 |
| 卷号 | 28页码:549-557 |
| 关键词 | Adipose-derived stem cells Three-dimensional cell culture Irradiation response High- and low-LET irradiation |
| ISSN号 | 0895-3988 |
| DOI | 10.3967/bes2015.079 |
| 英文摘要 | Objective Adipose tissue distributes widely in human body. The irradiation response of the adipose cells in vivo remains to be investigated. In this study we investigated irradiation response of adipose-derived stem cells (ASCs) under three-dimensional culture condition. Methods ASCs were isolated and cultured in low attachment dishes to form three-dimensional (3D) spheres in vitro. The neuronal differentiation potential and stem-liked characteristics was monitored by using immunofluoresence staining and flow cytometry in monolayer and 3D culture. To investigate the irradiation sensitivity of 3D sphere culture, the fraction of colony survival and micronucleus were detected in monolayer and 3D culture. Soft agar assays were performed for measuring malignant transformation for the irradiated monolayer and 3D culture. Results The 3D cultured ASCs had higher differentiation potential and an higher stem-like cell percentage. The 3D cultures were more radioresistant after either high linear energy transfer (LET) carbon ion beam or low LET X-ray irradiation compared with the monolayer cell. The ASCs' potential of cellular transformation was lower after irradiation by soft agar assay. Conclusion These findings suggest that adipose tissue cell are relatively genomic stable and resistant to genotoxic stress. |
| WOS关键词 | LOW ATTACHMENT SURFACES ; IN-VITRO ; EXTRACELLULAR-MATRIX ; STROMAL CELLS ; FORCED GROWTH ; 3D SPHERES ; TISSUE ; CANCER ; LINE ; RADIORESISTANCE |
| 资助项目 | National Key Scientific Instrument and Equipment Development Project of China[2012YQ03014210] ; Major Project Specialized for Infectious Diseases of the Chinese Health and Family Planning Commission[2014ZX10004002-004-002] ; National Natural Science Foundation of China[31170803] |
| WOS研究方向 | Environmental Sciences & Ecology ; Public, Environmental & Occupational Health |
| 语种 | 英语 |
| WOS记录号 | WOS:000361926400001 |
| 出版者 | CHINESE CENTER DISEASE CONTROL & PREVENTION |
| 资助机构 | National Key Scientific Instrument and Equipment Development Project of China ; Major Project Specialized for Infectious Diseases of the Chinese Health and Family Planning Commission ; National Natural Science Foundation of China |
| 源URL | [http://119.78.100.186/handle/113462/40774]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Hu Bu Rong |
| 作者单位 | 1.Chinese Acad Sci, Inst Modern Phys, Key Lab Heavy Ion Radiat Biol & Med, Gansu Key Lab Space Radiobiol, Lanzhou 730000, Gansu, Peoples R China 2.Sichuan Canc Hosp, Dept Radiat Oncol, Chengdu 610041, Sichuan, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Du Ya Rong,Pan Dong,Chen Ya Xiong,et al. Irradiation Response of Adipose-derived Stem Cells under Three-dimensional Culture Condition[J]. BIOMEDICAL AND ENVIRONMENTAL SCIENCES,2015,28:549-557. |
| APA | Du Ya Rong.,Pan Dong.,Chen Ya Xiong.,Xue Gang.,Ren Zhen Xin.,...&Hu Bu Rong.(2015).Irradiation Response of Adipose-derived Stem Cells under Three-dimensional Culture Condition.BIOMEDICAL AND ENVIRONMENTAL SCIENCES,28,549-557. |
| MLA | Du Ya Rong,et al."Irradiation Response of Adipose-derived Stem Cells under Three-dimensional Culture Condition".BIOMEDICAL AND ENVIRONMENTAL SCIENCES 28(2015):549-557. |
入库方式： OAI收割
来源：近代物理研究所
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