The developmental toxicity and apoptosis in zebrafish eyes induced by carbon-ion irradiation
文献类型：期刊论文
| 作者 | Zhou, Rong1,2; Zhang, Hong1,2 ; Wang, Zhenguo3; Zhou, Xin1,2; Si, Jing1,2; Gan, Lu1,2; Li, Jianzhen4; Liu, Yang1,2
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| 刊名 | LIFE SCIENCES
 |
| 出版日期 | 2015-10-15 |
| 卷号 | 139页码:114-122 |
| 关键词 | Carbon-ion beams Zebrafish Eye development Apoptosis Microphthalmia |
| ISSN号 | 0024-3205 |
| DOI | 10.1016/j.lfs.2015.08.014 |
| 英文摘要 | Aims: Heavy ions have become potentially radiotherapeutic tools. However, studies of the effects on development of normal organs were limited. Using a zebrafish model, this study investigated the potential developmental toxicity and cell apoptosis rates in eyes exposed to carbon-ion irradiation. Main methods: Zebrafish embryos at 12 h post-fertilization (hpf) were irradiated using C-12(6+) ion beams at doses of 2, 4, and 8 Gy. The reactive oxygen species (ROS) concentration was detected using the dichlorofluorescein-diacetate at 24, 48, and 72 hpf. Apoptosis was assessed by acridine orange staining at 24, 48, and 72 hpf and was also detected using the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling assay, at 72 hpf. The expression of genes governing apoptosis was examined using real-time polymerase chain reaction at 24 hpf. Eye size was measured at 144 hpf. Key findings: Ion irradiation with C-12(6+) induced a significant increase in cell apoptosis at 24,48 and 72 hpf. However, there was no significant increase in the ROS concentration at 24, 48, and 72 hpf. The proapoptotic genes, including P53, Box, and Puma, were significantly upregulated. Two antiapoptotic genes, Mdm2 and Bcl-2, were significantly downregulated, and the expression levels of Capspase-9 and Caspase-3 were significantly increased. Microphthalmia was noted in the 8 Gy irradiated group. Significance: These results suggested that carbon-ion irradiation induced apoptosis through the p53 pathway in zebrafish eyes independent of ROS generation. Irradiation at high doses may disrupt eye development of zebrafish embryos. (C) 2015 Elsevier Inc All rights reserved. |
| WOS关键词 | IONIZING-RADIATION ; OXIDATIVE STRESS ; DNA-DAMAGE ; CELL-DEATH ; IN-VIVO ; X-RAY ; VERTEBRATE EMBRYO ; GENE-EXPRESSION ; MODEL ORGANISM ; REPAIR |
| 资助项目 | National Natural Science Foundation of China[U1432248] ; National Natural Science Foundation of China[11305226] ; Scientific Technology Research Projects of Gansu Province[0702NKDA045] ; Scientific Technology Research Projects of Gansu Province[0806RJYA020] |
| WOS研究方向 | Research & Experimental Medicine ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000363710100016 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 资助机构 | National Natural Science Foundation of China ; Scientific Technology Research Projects of Gansu Province |
| 源URL | [http://119.78.100.186/handle/113462/40911]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Zhang, Hong |
| 作者单位 | 1.Chinese Acad Sci, Inst Modern Phys, Dept Heavy Ion Irradiat Med, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Key Lab Heavy Ion Irradiat Biol & Med, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Sch Hosp Stomatol, Lanzhou 730000, Peoples R China 4.Northwest Normal Univ, Coll Life Sci, Lanzhou, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Rong,Zhang, Hong,Wang, Zhenguo,et al. The developmental toxicity and apoptosis in zebrafish eyes induced by carbon-ion irradiation[J]. LIFE SCIENCES,2015,139:114-122. |
| APA | Zhou, Rong.,Zhang, Hong.,Wang, Zhenguo.,Zhou, Xin.,Si, Jing.,...&Liu, Yang.(2015).The developmental toxicity and apoptosis in zebrafish eyes induced by carbon-ion irradiation.LIFE SCIENCES,139,114-122. |
| MLA | Zhou, Rong,et al."The developmental toxicity and apoptosis in zebrafish eyes induced by carbon-ion irradiation".LIFE SCIENCES 139(2015):114-122. |
入库方式： OAI收割
来源：近代物理研究所
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