Downregulation of Nrf2 promotes radiation-induced apoptosis through Nrf2 mediated Notch signaling in non-small cell lung cancer cells
文献类型：期刊论文
| 作者 | Zhao, Qiuyue1,2,3,4; Mao, Aihong1,2,3,4; Yan, Jiawei1,2,3,4; Sun, Chao1,2,3; Di, Cuixia1,2,3 ; Zhou, Xin1,2,3; Li, Hongyan1,2,3; Guo, Ruoshui5; Zhang, Hong1,2,3
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| 刊名 | INTERNATIONAL JOURNAL OF ONCOLOGY
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| 出版日期 | 2016-02-01 |
| 卷号 | 48页码:765-773 |
| 关键词 | nuclear factor erythroid-2-related factor 2 ionizing radiation ROS Notch1 lung cancer |
| ISSN号 | 1019-6439 |
| DOI | 10.3892/ijo.2015.3301 |
| 英文摘要 | The nuclear factor erythroid-2-related factor 2 (Nrf2) is a crucial regulator of the cellular antioxidant system. Nrf2 is often constitutively activated in non-small cell lung cancer (NSCLC) cell lines, which promotes cytoprotection against oxidative stress and xenobiotics. Notch1 signaling is critically implicated in cell fate determination. It has been reported that Nf2 strongly regulates Notch1 activity. However, the role of Nrf2 mediated Notch1 signaling in response to ionizing radiation (IR) remains elusive. We report that knockdown of Nrf2 promotes radiation-induced apoptosis through Nrf2 mediated Notch1 signaling in NSCLC cells. IR activated Nrf2 in a dose-dependent manner and the expression of Nrf2 was significantly elevated at 4 h after exposure. RNAi-mediated reduction of Nrf2 significantly increased endogenous ROS levels, and decreased the expression of glutamate cysteine ligase catalytic subunit (GCLC), heme oxygenase-1 (HO-1) and NAD (P) H quinine oxidoreductase-1 (NQO1) in irradiated cells. Furthermore, decrease in Nrf2 expression significantly dampened Notch1 expression following ionizing radiation exposure, and potentiated IR-induced cellular apoptosis. These results demonstrated that Nrf2 could be activated by ionizing radiation, knockdown of Nrf2 could promote radiation induced apoptosis and Nrf2-mediated Notch signaling is an important determinant in radioresistance of lung cancer cells. |
| WOS关键词 | GENE-EXPRESSION ; HEPATOCELLULAR-CARCINOMA ; NRF2/KEAP1 PATHWAY ; SYSTEMIC-SCLEROSIS ; STEM-CELLS ; IN-VIVO ; ACTIVATION ; SURVIVAL ; RADIORESISTANCE ; GROWTH |
| 资助项目 | Key Program of National Natural Science Foundation of China[U1432248] ; National Natural Science Foundation of China[11105203] ; National Natural Science Foundation of China[11305224] |
| WOS研究方向 | Oncology |
| 语种 | 英语 |
| WOS记录号 | WOS:000366897500033 |
| 出版者 | SPANDIDOS PUBL LTD |
| 资助机构 | Key Program of National Natural Science Foundation of China ; National Natural Science Foundation of China |
| 源URL | [http://119.78.100.186/handle/113462/41274]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Zhang, Hong |
| 作者单位 | 1.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Gansu, Peoples R China 2.Chinese Acad Sci, Key Lab Heavy Ion Radiat Med, Lanzhou 730000, Gansu, Peoples R China 3.Key Lab Heavy Ion Radiat Med Gansu Prov, Lanzhou 730000, Gansu, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 5.South China Agr Univ, Guangzhou 510642, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Qiuyue,Mao, Aihong,Yan, Jiawei,et al. Downregulation of Nrf2 promotes radiation-induced apoptosis through Nrf2 mediated Notch signaling in non-small cell lung cancer cells[J]. INTERNATIONAL JOURNAL OF ONCOLOGY,2016,48:765-773. |
| APA | Zhao, Qiuyue.,Mao, Aihong.,Yan, Jiawei.,Sun, Chao.,Di, Cuixia.,...&Zhang, Hong.(2016).Downregulation of Nrf2 promotes radiation-induced apoptosis through Nrf2 mediated Notch signaling in non-small cell lung cancer cells.INTERNATIONAL JOURNAL OF ONCOLOGY,48,765-773. |
| MLA | Zhao, Qiuyue,et al."Downregulation of Nrf2 promotes radiation-induced apoptosis through Nrf2 mediated Notch signaling in non-small cell lung cancer cells".INTERNATIONAL JOURNAL OF ONCOLOGY 48(2016):765-773. |
入库方式： OAI收割
来源：近代物理研究所
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