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Both Complexity and Location of DNA Damage Contribute to Cellular Senescence Induced by Ionizing Radiation

文献类型：期刊论文

作者Zhang, Xurui1,2,3; Ye, Caiyong1,2; Sun, Fang1,2,3; Wei, Wenjun1,2,3; Hu, Burong1,2; Wang, Jufang1,2
刊名PLOS ONE
出版日期2016-05-17
卷号11页码:16
ISSN号1932-6203
DOI10.1371/journal.pone.0155725
英文摘要Persistent DNA damage is considered as a main cause of cellular senescence induced by ionizing radiation. However, the molecular bases of the DNA damage and their contribution to cellular senescence are not completely clear. In this study, we found that both heavy ions and X-rays induced senescence in human uveal melanoma 92-1 cells. By measuring senescence associated-beta-galactosidase and cell proliferation, we identified that heavy ions were more effective at inducing senescence than X-rays. We observed less efficient repair when DNA damage was induced by heavy ions compared with X-rays and most of the irreparable damage was complex of single strand breaks and double strand breaks, while DNA damage induced by X-rays was mostly repaired in 24 hours and the remained damage was preferentially associated with telomeric DNA. Our results suggest that DNA damage induced by heavy ion is often complex and difficult to repair, thus presents as persistent DNA damage and pushes the cell into senescence. In contrast, persistent DNA damage induced by X-rays is preferentially associated with telomeric DNA and the telomere-favored persistent DNA damage contributes to X-rays induced cellular senescence. These findings provide new insight into the understanding of high relative biological effectiveness of heavy ions relevant to cancer therapy and space radiation research.
WOS关键词DOUBLE-STRAND BREAKS ; CELLS ; REPAIR ; HETEROCHROMATIN ; FIBROBLASTS ; MELANOMA ; PATHWAY ; ARREST ; CANCER ; ATM
资助项目National Natural Science Foundation of China[U1232125] ; National Natural Science Foundation of China[31270895] ; National Natural Science Foundation of China[U1432121]
WOS研究方向Science & Technology - Other Topics
语种英语
WOS记录号WOS:000376282300063
出版者PUBLIC LIBRARY SCIENCE
资助机构National Natural Science Foundation of China
源URL[http://119.78.100.186/handle/113462/42671]  
专题中国科学院近代物理研究所
通讯作者Wang, Jufang
作者单位1.Chinese Acad Sci, Gansu Key Lab Space Radiobiol, Inst Modern Phys, Lanzhou 730000, Peoples R China
2.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Inst Modern Phys, Lanzhou 730000, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Xurui,Ye, Caiyong,Sun, Fang,et al. Both Complexity and Location of DNA Damage Contribute to Cellular Senescence Induced by Ionizing Radiation[J]. PLOS ONE,2016,11:16.
APA Zhang, Xurui,Ye, Caiyong,Sun, Fang,Wei, Wenjun,Hu, Burong,&Wang, Jufang.(2016).Both Complexity and Location of DNA Damage Contribute to Cellular Senescence Induced by Ionizing Radiation.PLOS ONE,11,16.
MLA Zhang, Xurui,et al."Both Complexity and Location of DNA Damage Contribute to Cellular Senescence Induced by Ionizing Radiation".PLOS ONE 11(2016):16.

入库方式： OAI收割

来源：近代物理研究所

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