Genistein sensitizes sarcoma cells in vitro and in vivo by enhancing apoptosis and by inhibiting DSB repair pathways
文献类型：期刊论文
| 作者 | Liu, X. X.1,2,3; Sun, C.1,2,3; Jin, X. D.1,2,3; Li, P.1,2,3 ; Zheng, X. G.1,2,3,4; Zhao, T.1,2,3; Li, Q.1,2,3
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| 刊名 | JOURNAL OF RADIATION RESEARCH
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| 出版日期 | 2016-06-01 |
| 卷号 | 57页码:227-237 |
| 关键词 | sarcoma radiosensitivity genistein DNA damage repair apoptosis |
| ISSN号 | 0449-3060 |
| DOI | 10.1093/jrr/rrv091 |
| 英文摘要 | The aim of this work was to investigate the radiosensitization effects of genistein on mice sarcoma cells and the corresponding biological mechanisms in vitro and in vivo. Using the non-toxic dosage of 10 mu M genistein, the sensitizer enhancement ratios after exposure to X-rays at 50% cell survival (IC50) was 1.45 for S180 cells. For mice cotreated with genistein and X-rays, the excised tumor tissues had reduced blood vessels and decreased size and volume compared with the control and irradiation-only groups. Moreover, a significant increase in apoptosis was accompanied by upregulation of Bax and downregulation of Bcl-2 in the mitochondria, and lots of cytochrome c being transferred to the cytoplasm. Furthermore, X-rays combined with genistein inhibited the activity of DNA-PKcs, so DNA-injured sites were dominated by Ku70/80, leading to incompleteness of homologous recombination (HR) and non-homologous end-joining (NHEJ) repairs and the eventual occurrence of cell apoptosis. Our study, for the first time, demonstrated that genistein sensitized sarcoma cells to X-rays and that this radiosensitizing effect depended on induction of the mitochondrial apoptosis pathway and inhibition of the double-strand break (DSB) repair pathways. |
| WOS关键词 | DEPENDENT PROTEIN-KINASE ; BREAST-CANCER CELLS ; PROSTATE-CANCER ; CATALYTIC SUBUNIT ; HOMOLOGOUS RECOMBINATION ; MAMMALIAN-CELLS ; DNA-REPAIR ; RADIOSENSITIVITY ; RADIATION ; OSTEOSARCOMA |
| 资助项目 | Western Talent Program of the Chinese Academy of Sciences[Y562020XB0] |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics ; Radiology, Nuclear Medicine & Medical Imaging |
| 语种 | 英语 |
| WOS记录号 | WOS:000378758100004 |
| 出版者 | OXFORD UNIV PRESS |
| 资助机构 | Western Talent Program of the Chinese Academy of Sciences |
| 源URL | [http://119.78.100.186/handle/113462/43004]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Li, Q. |
| 作者单位 | 1.Chinese Acad Sci, Inst Modern Phys, 509 Nanchang Rd, Lanzhou 730000, Gansu, Peoples R China 2.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, 509 Nanchang Rd, Lanzhou 730000, Gansu, Peoples R China 3.Key Lab Basic Res Heavy Ion Radiat Applicat Med, Lanzhou 730000, Gansu, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, X. X.,Sun, C.,Jin, X. D.,et al. Genistein sensitizes sarcoma cells in vitro and in vivo by enhancing apoptosis and by inhibiting DSB repair pathways[J]. JOURNAL OF RADIATION RESEARCH,2016,57:227-237. |
| APA | Liu, X. X..,Sun, C..,Jin, X. D..,Li, P..,Zheng, X. G..,...&Li, Q..(2016).Genistein sensitizes sarcoma cells in vitro and in vivo by enhancing apoptosis and by inhibiting DSB repair pathways.JOURNAL OF RADIATION RESEARCH,57,227-237. |
| MLA | Liu, X. X.,et al."Genistein sensitizes sarcoma cells in vitro and in vivo by enhancing apoptosis and by inhibiting DSB repair pathways".JOURNAL OF RADIATION RESEARCH 57(2016):227-237. |
入库方式： OAI收割
来源：近代物理研究所
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