Molecular Characterization of LRB7 Gene and a Water Channel Protein TIP2 in Chorispora bungeana
文献类型：期刊论文
| 作者 | Li, Ming1,2; Liang, Zhaoxu1; Di, Cuixia3 ; Fang, Weikuan4; Wu, Kaichao1; Chen, Maoshan5; He, Shanshan1; Zeng, Yuan4; Jing, Yan1; Liang, Jun1
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| 刊名 | BIOMED RESEARCH INTERNATIONAL
 |
| 出版日期 | 2016 |
| 页码 | 11 |
| ISSN号 | 2314-6133 |
| DOI | 10.1155/2016/2483258 |
| 英文摘要 | Background. Water channel proteins, also called a quaporins, are integral membrane proteins from major intrinsic protein (MIP) family and involved in several pathways including not only water transport but also cell signaling, reproduction, and photosynthesis. The full cDNA and protein sequences of aquaporin in Chorispora bungeana Fisch. & C.A. Mey (C. bungeana) are still unknown. Results. In this study, PCR and rapid amplification of cDNA ends approaches were used to clone the full cDNA of LRB7 (GenBank accession number: EU636988) of C. bungeana. Sequence analysis indicated that it was 1235 bp, which had two introns and encoded a protein of 250 amino acids. Structure analysis revealed that the protein had two conserved NPA motifs, one of which is MIP signature sequence (SGxHxNPAVT), six membrane helix regions, and additional membrane-embedded domains. Phylogenetic analysis suggested that the protein was from TIP2 subgroup. Surprisingly, semiquantitative RT-PCR experiment and western blot analysis showed that LRB7 and TIP2 were only detectable in roots, unlike Arabidopsis and Raphanus. Connecting with our previous studies, LRB7 was supported to associate with chilling-tolerance in C. bungeana. Conclusion. This is the first time to characterize the full sequences of LRB7 gene and water channel protein in C. bungeana. Our findings contribute to understanding the water transports in plants under low temperatures. |
| WOS关键词 | SUSPENSION-CULTURED CELLS ; MAJOR INTRINSIC PROTEINS ; CHILLING TOLERANCE ; XENOPUS-OOCYTES ; PLANT AQUAPORINS ; PLASMA-MEMBRANE ; EXPRESSION ; CLONING ; FAMILY ; MERCURY |
| 资助项目 | National Science Foundation of China[31460093] ; National Science Foundation of China[31400281] ; Guangxi Scientific Research and Technology Development Plan[GuiKeZhong 14121005-1-2] ; Guangxi Scientific Research and Technology Development Plan[GuiKeGong 1598006-1-1D] ; Nanning Scientific Research and Technology Development Plan[20131038] ; Development Foundation of Guangxi Academy of Agricultural Science[2015JZ15] ; Development Foundation of Guangxi Academy of Agricultural Science[2015JZ91] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Research & Experimental Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:000383094400001 |
| 出版者 | HINDAWI PUBLISHING CORP |
| 资助机构 | National Science Foundation of China ; Guangxi Scientific Research and Technology Development Plan ; Nanning Scientific Research and Technology Development Plan ; Development Foundation of Guangxi Academy of Agricultural Science |
| 源URL | [http://119.78.100.186/handle/113462/43206]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | An, Lizhe |
| 作者单位 | 1.Guangxi Acad Agr Sci, Sugarcane Res Inst, Nanning 530007, Peoples R China 2.Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China 4.Guangxi Acad Agr Sci, Nanning 530007, Peoples R China 5.La Trobe Univ, La Trobe Inst Mol Sci, Dept Biochem & Genet, Melbourne, Vic 3086, Australia 6.Lanzhou Univ, State Key Lab Arid Agroecol, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Ming,Liang, Zhaoxu,Di, Cuixia,et al. Molecular Characterization of LRB7 Gene and a Water Channel Protein TIP2 in Chorispora bungeana[J]. BIOMED RESEARCH INTERNATIONAL,2016:11. |
| APA | Li, Ming.,Liang, Zhaoxu.,Di, Cuixia.,Fang, Weikuan.,Wu, Kaichao.,...&An, Lizhe.(2016).Molecular Characterization of LRB7 Gene and a Water Channel Protein TIP2 in Chorispora bungeana.BIOMED RESEARCH INTERNATIONAL,11. |
| MLA | Li, Ming,et al."Molecular Characterization of LRB7 Gene and a Water Channel Protein TIP2 in Chorispora bungeana".BIOMED RESEARCH INTERNATIONAL (2016):11. |
入库方式： OAI收割
来源：近代物理研究所
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