Comparative proteomics reveals the underlying toxicological mechanism of low sperm motility induced by iron ion radiation in mice
文献类型：期刊论文
| 作者 | Li, Hongyan1,2,3; He, Yuxuan4; Yan, Jiawei1,2,3,5; Zhao, Qiuyue1,2,3; Di, Cuixia1,2,3 ; Zhang, Hong1,2,3
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| 刊名 | REPRODUCTIVE TOXICOLOGY
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| 出版日期 | 2016-10-01 |
| 卷号 | 65页码:148-158 |
| 关键词 | Proteomics Sperm Motility Space radiation |
| ISSN号 | 0890-6238 |
| DOI | 10.1016/j.reprotox.2016.07.014 |
| 英文摘要 | The toxicological mechanism of low sperm motility induced by iron ion radiation (IIR) was investigated in mice. Reproductive organ indices were measured following whole-body irradiation with a 2 Gy iron ion beam. Two-dimensional gel electrophoresis, immunoblotting and immunofluorescence were used to analyze protein expression, and real-time polymerase chain reaction was performed to confirm altered gene expression. Reproductive organ indices and sperm motility were lowest 2 weeks after IIR. Sperm function changes via testis and cauda epididymis function were also determined at this time point. Sixteen differentially expressed proteins were identified in sperm 2 weeks after IIR. Bioinformatics analysis indicated that alpha-enolase (Enol) may be important in the regulation of glycolysis in sperm, and Enol expression was correlated with sperm motility. Enol may be a potential marker for low sperm motility induced by IIR, and these results may provide a useful reference for changes in astronaut fertility during long space missions. (C) 2016 Elsevier Inc. All rights reserved. |
| WOS关键词 | CREATINE-KINASE ; GFAP EXPRESSION ; SPACE RADIATION ; MOUSE TESTIS ; CELL-LINE ; IN-VITRO ; SPERMATOZOA ; MEN ; INFERTILITY ; IRRADIATION |
| 资助项目 | National Natural Science Foundation of China[Y660010GJ0] ; National Natural Science Foundation of China[U1432248] ; Western Talent Program of the Chinese Academy of Sciences[Y560020XB0] ; Ministry of Science and Technology National Key RD project[2016YFC0904600] ; Fostering Foundation for the Excellent Ph.D. Dissertation of Gansu Agricultural University[2013002] |
| WOS研究方向 | Reproductive Biology ; Toxicology |
| 语种 | 英语 |
| WOS记录号 | WOS:000385990900018 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 资助机构 | National Natural Science Foundation of China ; Western Talent Program of the Chinese Academy of Sciences ; Ministry of Science and Technology National Key RD project ; Fostering Foundation for the Excellent Ph.D. Dissertation of Gansu Agricultural University |
| 源URL | [http://119.78.100.186/handle/113462/43571]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Zhang, Hong |
| 作者单位 | 1.Chinese Acad Sci, Inst Modern Phys, Dept Radiat Med, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Peoples R China 3.Key Lab Basic Res Heavy Ion Radiat Applicat Med, Lanzhou 730000, Peoples R China 4.Gansu Agr Univ, Coll Vet Med, Lanzhou 730070, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Hongyan,He, Yuxuan,Yan, Jiawei,et al. Comparative proteomics reveals the underlying toxicological mechanism of low sperm motility induced by iron ion radiation in mice[J]. REPRODUCTIVE TOXICOLOGY,2016,65:148-158. |
| APA | Li, Hongyan,He, Yuxuan,Yan, Jiawei,Zhao, Qiuyue,Di, Cuixia,&Zhang, Hong.(2016).Comparative proteomics reveals the underlying toxicological mechanism of low sperm motility induced by iron ion radiation in mice.REPRODUCTIVE TOXICOLOGY,65,148-158. |
| MLA | Li, Hongyan,et al."Comparative proteomics reveals the underlying toxicological mechanism of low sperm motility induced by iron ion radiation in mice".REPRODUCTIVE TOXICOLOGY 65(2016):148-158. |
入库方式： OAI收割
来源：近代物理研究所
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