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Carbon Ion-Irradiated Hepatoma Cells Exhibit Coupling Interplay between Apoptotic Signaling and Morphological and Mechanical Remodeling

文献类型：期刊论文

作者Zhang, Baoping1,2,3; Li, Long1,3; Li, Zhiqiang4; Liu, Yang2; Zhang, Hong2; Wang, Jizeng1,3
刊名SCIENTIFIC REPORTS
出版日期2016-10-12
卷号6页码:13
ISSN号2045-2322
DOI10.1038/srep35131
英文摘要A apoptotic model was established based on the results of five hepatocellular carcinoma cell (HCC) lines irradiated with carbon ions to investigate the coupling interplay between apoptotic signaling and morphological and mechanical cellular remodeling. The expression levels of key apoptotic proteins and the changes in morphological characteristics and mechanical properties were systematically examined in the irradiated HCC lines. We observed that caspase-3 was activated and that the Bax/Bcl-2 ratio was significantly increased over time. Cellular morphology and mechanics analyses indicated monotonic decreases in spatial sizes, an increase in surface roughness, a considerable reduction in stiffness, and disassembly of the cytoskeletal architecture. A theoretical model of apoptosis revealed that mechanical changes in cells induce the characteristic cellular budding of apoptotic bodies. Statistical analysis indicated that the projected area, stiffness, and cytoskeletal density of the irradiated cells were positively correlated, whereas stiffness and caspase-3 expression were negatively correlated, suggesting a tight coupling interplay between the cellular structures, mechanical properties, and apoptotic protein levels. These results help to clarify a novel arbitration mechanism of cellular demise induced by carbon ions. This biomechanics strategy for evaluating apoptosis contributes to our understanding of cancer-killing mechanisms in the context of carbon ion radiotherapy.
WOS关键词RELATIVE BIOLOGICAL EFFECTIVENESS ; CYTOSKELETAL DISRUPTION ; ACTIN CYTOSKELETON ; CASPASE ACTIVATION ; DEATH ; CANCER ; GELSOLIN ; BEAMS ; MECHANOTRANSDUCTION ; AUTOPHAGY
资助项目National Natural Science Foundation of China[11032006] ; National Natural Science Foundation of China[11121202] ; National Natural Science Foundation of China[11472119] ; National Natural Science Foundation of China[11421062] ; National Natural Science Foundation of China[11305224] ; National Natural Science Foundation of China[11575262]
WOS研究方向Science & Technology - Other Topics
语种英语
WOS记录号WOS:000384991500001
出版者NATURE PUBLISHING GROUP
资助机构National Natural Science Foundation of China
源URL[http://119.78.100.186/handle/113462/43628]  
专题中国科学院近代物理研究所
通讯作者Liu, Yang; Wang, Jizeng
作者单位1.Lanzhou Univ, Coll Civil Engn & Mech, Key Lab Mech Disaster & Environm Western China, Minist Educ, Lanzhou 730000, Peoples R China
2.Chinese Acad Sci, Inst Modern Phys, Dept Heavy Radiat Med, Lanzhou 730000, Peoples R China
3.Lanzhou Univ, Inst Biomech & Med Engn, Lanzhou 730000, Peoples R China
4.Northwest Univ Nationalities, Key Lab Oral Dis Gansu Prov, Lanzhou 730030, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Baoping,Li, Long,Li, Zhiqiang,et al. Carbon Ion-Irradiated Hepatoma Cells Exhibit Coupling Interplay between Apoptotic Signaling and Morphological and Mechanical Remodeling[J]. SCIENTIFIC REPORTS,2016,6:13.
APA Zhang, Baoping,Li, Long,Li, Zhiqiang,Liu, Yang,Zhang, Hong,&Wang, Jizeng.(2016).Carbon Ion-Irradiated Hepatoma Cells Exhibit Coupling Interplay between Apoptotic Signaling and Morphological and Mechanical Remodeling.SCIENTIFIC REPORTS,6,13.
MLA Zhang, Baoping,et al."Carbon Ion-Irradiated Hepatoma Cells Exhibit Coupling Interplay between Apoptotic Signaling and Morphological and Mechanical Remodeling".SCIENTIFIC REPORTS 6(2016):13.

入库方式： OAI收割

来源：近代物理研究所

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