A novel peptide-based fluorescence chemosensor for selective imaging of hydrogen sulfide both in living cells and zebrafish
文献类型：期刊论文
| 作者 | Wang, Peng1,2; Wu, Jiang1,2; Di, Cuixia3 ; Zhou, Rong3; Zhang, Hong3; Su, Pingru1,2; Xu, Cong1,2; Zhou, Panpan1,2; Ge, Yushu4; Li, Dan4
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| 刊名 | BIOSENSORS & BIOELECTRONICS
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| 出版日期 | 2017-06-15 |
| 卷号 | 92页码:602-609 |
| 关键词 | Peptide Fluorescence chemosensor Hydrogen sulfide detection Cell imaging Zebrafish imaging |
| ISSN号 | 0956-5663 |
| DOI | 10.1016/j.bios.2016.10.050 |
| 英文摘要 | Hydrogen sulfide (H2S) plays an important role as a signaling compound (gasotransmitter) in living systems. However, the development of an efficient imaging chemosensor of H2S in live animals is a challenging field for chemists. Herein, a novel peptide-based fluorescence chemosensor L-Cu was designed and synthesized on the basis of the copper chelating with the peptide ligand (FITC-Ahx-Ser-Pro-Gly-His-NH2, L), and its H2S sensing ability has been evaluated both in living cells and zebrafish. The peptide backbone and Cu2+-removal sensing mechanism are used to deliver rapid response time, high sensitivity, and good biocompatibility. After a fast fluorescence quench by Cu2+ coordinated with L, the fluorescence of L is recovered by adding S2- to form insoluble copper sulfide in aqueous solution with a detection limit for hydrogen sulfide measured to be 31 nM. Furthermore, the fluorescence chemosensor L-Cu showed excellent cell permeation and low biotoxicity to realize the intracellular biosensing, L-Cu has also been applied to image hydrogen sulfide in live zebrafish larvae. We expect that this peptide-based fluorescence chemosensor L-Cu can be used to study H2S-related chemical biology in physiological and pathological events. |
| WOS关键词 | LIVE CELLS ; BIOLOGICAL-SYSTEMS ; AQUEOUS-SOLUTIONS ; ZINC IONS ; PROBE ; SENSORS ; DESIGN ; H2S ; LUMINESCENCE ; INDICATOR |
| 资助项目 | National Natural Science Foundation of China[21471071] ; National Natural Science Foundation of China[21431002] ; National Natural Science Foundation of China[21201091] ; Fundamental Research Funds for the Central Universities[lzujbky-2016-k10] ; Fundamental Research Funds for the Central Universities[lzujbky-2016-211] |
| WOS研究方向 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000396946600083 |
| 出版者 | ELSEVIER ADVANCED TECHNOLOGY |
| 资助机构 | National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities |
| 源URL | [http://119.78.100.186/handle/113462/44643]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Wu, Jiang; Tang, Yu |
| 作者单位 | 1.Lanzhou Univ, Coll Chem & Chem Engn, Key Lab Nonferrous Met Chem & Resources Utilizat, Lanzhou 730000, Peoples R China 2.Lanzhou Univ, Coll Chem & Chem Engn, State Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Inst Modern Phys, Dept Heavy Ion Radiat Med, 509 Nanchang Rd, Lanzhou 730000, Peoples R China 4.Univ Sci & Technol China, Sch Life Sci, Collaborat Innovat Ctr Chem Life Sci, Hefei 230027, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Peng,Wu, Jiang,Di, Cuixia,et al. A novel peptide-based fluorescence chemosensor for selective imaging of hydrogen sulfide both in living cells and zebrafish[J]. BIOSENSORS & BIOELECTRONICS,2017,92:602-609. |
| APA | Wang, Peng.,Wu, Jiang.,Di, Cuixia.,Zhou, Rong.,Zhang, Hong.,...&Tang, Yu.(2017).A novel peptide-based fluorescence chemosensor for selective imaging of hydrogen sulfide both in living cells and zebrafish.BIOSENSORS & BIOELECTRONICS,92,602-609. |
| MLA | Wang, Peng,et al."A novel peptide-based fluorescence chemosensor for selective imaging of hydrogen sulfide both in living cells and zebrafish".BIOSENSORS & BIOELECTRONICS 92(2017):602-609. |
入库方式： OAI收割
来源：近代物理研究所
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