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Label-free fluorescence imaging of cytochrome c in living systems and anti-cancer drug screening with nitrogen doped carbon quantum dots

文献类型：期刊论文

作者Zhang, Haijuan1; Zhang, Baoxin2,3; Di, Cuixia4; Ali, Mohammad Chand1; Chen, Jia1; Li, Zhan1; Si, Jing4; Zhang, Hong4; Qiu, Hongdeng1
刊名NANOSCALE
出版日期2018-03-21
卷号10页码:5342-5349
ISSN号2040-3364
DOI10.1039/c7nr08987b
英文摘要As an important biomarker for the early stage of apoptosis, cytochrome c (Cyt c) has been recognized as a key component of the intrinsic apoptotic pathway. Fluorescence imaging tools enabling detection of Cyt c in apoptotic signaling have been rarely explored, though they are critical for cell biology and clinical theranostics. Here, we designed a novel label-free N-doped carbon dot (N-doped CD)-based nanosensor that enables fluorescence activation imaging of Cyt c release in cell apoptosis. The inner filter effect of Cyt c towards N-doped CDs enabled quantitative Cyt c measurement. The nanosensor exhibited high sensitivity and selectivity, rapid response, good cell-membrane permeability and low cytotoxicity. All these features are favorable for in situ visualization of Cyt c for apoptosis research. Notably, the developed nanosensor was successfully applied to monitor intracellular release of Cyt c, and to visualize Cyt c in living zebrafish for the first time. Moreover, it also provided a viable platform for cell-based screening of apoptosis-inducing compounds. In virtue of these advantages and potential, the developed assay not only holds great significance for the better understanding of certain diseases at the cellular level, but also provides an invaluable platform for apoptotic studies and screening of anti-cancer drugs toward drug development.
WOS关键词SELECTIVE DETECTION ; MEMBRANE PERMEABILIZATION ; THERAPEUTIC-EFFICACY ; AQUEOUS-SOLUTION ; GREEN SYNTHESIS ; CELLS ; APOPTOSIS ; MITOCHONDRIA ; NANOPARTICLES ; EMISSION
资助项目National Natural Science Foundation of China[21475142] ; National Natural Science Foundation of China[21675164] ; National Natural Science Foundation of China[21650110454] ; Natural Science Foundation of Gansu Province[1506RJDA281] ; Natural Science Foundation of Gansu Province[17JR5RA311]
WOS研究方向Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
语种英语
WOS记录号WOS:000428787200039
出版者ROYAL SOC CHEMISTRY
资助机构National Natural Science Foundation of China ; Natural Science Foundation of Gansu Province
源URL[http://119.78.100.186/handle/113462/46481]  
专题中国科学院近代物理研究所
通讯作者Qiu, Hongdeng
作者单位1.Chinese Acad Sci, Lanzhou Inst Chem Phys, CAS Key Lab Chem Northwestern Plant Resources, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Gansu, Peoples R China
2.Lanzhou Univ, State Key Lab Appl Organ Chem, Lanzhou 730000, Gansu, Peoples R China
3.Lanzhou Univ, Coll Chem & Chem Engn, Lanzhou 730000, Gansu, Peoples R China
4.Chinese Acad Sci, Inst Modern Phys, Dept Radiat Med, Lanzhou 730000, Gansu, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Haijuan,Zhang, Baoxin,Di, Cuixia,et al. Label-free fluorescence imaging of cytochrome c in living systems and anti-cancer drug screening with nitrogen doped carbon quantum dots[J]. NANOSCALE,2018,10:5342-5349.
APA Zhang, Haijuan.,Zhang, Baoxin.,Di, Cuixia.,Ali, Mohammad Chand.,Chen, Jia.,...&Qiu, Hongdeng.(2018).Label-free fluorescence imaging of cytochrome c in living systems and anti-cancer drug screening with nitrogen doped carbon quantum dots.NANOSCALE,10,5342-5349.
MLA Zhang, Haijuan,et al."Label-free fluorescence imaging of cytochrome c in living systems and anti-cancer drug screening with nitrogen doped carbon quantum dots".NANOSCALE 10(2018):5342-5349.

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来源：近代物理研究所

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