Effects of C-12(6+) heavy ion beam irradiation on the p53 signaling pathway in HepG2 liver cancer cells
文献类型：期刊论文
| 作者 | Liu, Kai1,2; Zhao, Xinke1,2; Gu, Jing2; Wu, Jianjun2; Zhang, Hong3; Li, Yingdong1,2,3 |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2017-11-01 |
| 卷号 | 49页码:989-998 |
| 关键词 | C-12(6+) heavy ion beam HepG2 p53 signaling pathway apoptosis |
| ISSN号 | 1672-9145 |
| DOI | 10.1093/abbs/gmx096 |
| 英文摘要 | The heavy ion beam is considered to be the ideal source for radiotherapy. The p53 tumor suppressor gene senses DNA damage and transducts intracellular apoptosis signals. Previous reports showed that the heavy ion beam can trigger complex forms of damage to cellular DNA, leading to cell cycle arrest and apoptosis of HepG2 human liver cancer cells; however, the mechanisms remains unclear fully. In order to explore whether the intrinsic or extrinsic pathway participates this process, HepG2 cells were treated with C-12(6+) HIB irradiation at doses of 0 (control), 1, 2, 4, and 6 Gy with various methods employed to understand relevant mechanisms, such as detection of apoptosis, cell cycle, and Fas expression by flow cytometry, analysis of apoptotic morphology by electron microscopy and laser scanning confocal microscopy, and screening differentially expressed genes relating to p53 signaling pathway by PCR-array assay following with any genes confirmed by western blot analysis. This study showed that C-12(6+) heavy ion beam irradiation at a dose of 6 Gy leads to endogenous DNA double-strand damage, G2/M cell cycle arrest, and apoptosis of human HepG2 cells via synergistic effect of the extrinsic and intrinsic pathways. Differentially expressed genes in the p53 signaling pathway related to DNA damage repair, apoptosis, cycle regulation, metastasis, deterioration and radioresistance were also discovered. Consequently, the expressions of Fas, TP53BP2, TP53AIP1, and CASP9 were confirmed upregulated after C-12(6+) HIB irradiation treatment. In conclusion, this study demonstrated the mechanisms of inhibition and apoptosis induced by C-12(6+) heavy ion beam irradiation on HepG2 cancer cells is mediated by initiation of the biological function of p53 signaling pathway including extrinsic and intrinsic apoptosis pathway. |
| WOS关键词 | APOPTOSIS-STIMULATING PROTEIN ; DNA-DAMAGE ; BREAST-CANCER ; PROMOTES MIGRATION ; CYTOCHROME-C ; CYCLIN-G ; EXPRESSION ; RADIATION ; REPAIR ; CARCINOMA |
| 资助项目 | Science Program of the Natural Science Foundation of China[81160478] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000418269100004 |
| 出版者 | OXFORD UNIV PRESS |
| 资助机构 | Science Program of the Natural Science Foundation of China |
| 源URL | [http://119.78.100.186/handle/113462/47064]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Li, Yingdong |
| 作者单位 | 1.Lanzhou Univ, Sch Basic Med Sci, Lanzhou 730000, Gansu, Peoples R China 2.Gansu Univ Chinese Med, Lanzhou 730000, Gansu, Peoples R China 3.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Dept Heavy Ion Irradiat Med, Inst Modern Phys, Lanzhou 730000, Gansu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Kai,Zhao, Xinke,Gu, Jing,et al. Effects of C-12(6+) heavy ion beam irradiation on the p53 signaling pathway in HepG2 liver cancer cells[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2017,49:989-998. |
| APA | Liu, Kai,Zhao, Xinke,Gu, Jing,Wu, Jianjun,Zhang, Hong,&Li, Yingdong.(2017).Effects of C-12(6+) heavy ion beam irradiation on the p53 signaling pathway in HepG2 liver cancer cells.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,49,989-998. |
| MLA | Liu, Kai,et al."Effects of C-12(6+) heavy ion beam irradiation on the p53 signaling pathway in HepG2 liver cancer cells".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 49(2017):989-998. |
入库方式： OAI收割
来源：近代物理研究所
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