Three-dimensional Culture of Human Airway Epithelium in Matrigel for Evaluation of Human Rhinovirus C and Bocavirus Infections
文献类型：期刊论文
| 作者 | Chen Ya Xiong1,2; Xie Guang Cheng3; Pan Dong1,2; Du Ya Rong1,2; Pang Li Li4; Song Jing Dong4; Duan Zhao Jun4; Hu Bu Rong1,2 |
| 刊名 | BIOMEDICAL AND ENVIRONMENTAL SCIENCES
 |
| 出版日期 | 2018-02-01 |
| 卷号 | 31页码:136-145 |
| 关键词 | 3D cell culture Human airway epithelium (HAE) Human rhinovirus C Human bocavirus Propagation |
| ISSN号 | 0895-3988 |
| DOI | 10.3967/bes2018.016 |
| 英文摘要 | Objective Newly identified human rhinovirus C (HRV-C) and human bocavirus (HBoV) cannot propagate in vitro in traditional cell culture models; thus obtaining knowledge about these viruses and developing related vaccines are difficult. Therefore, it is necessary to develop a novel platform for the propagation of these types of viruses. Methods A platform for culturing human airway epithelia in a three-dimensional (3D) pattern using Matrigel as scaffold was developed. The features of 3D culture were identified by immunochemical staining and transmission electron microscopy. Nucleic acid levels of HRV-C and HBoV in 3D cells at designated time points were quantitated by real-time polymerase chain reaction (PCR). Levels of cytokines, whose secretion was induced by the viruses, were measured by ELISA. Results Properties of bronchial-like tissues, such as the expression of biomarkers CK5, ZO-1, and PCK, and the development of cilium-like protuberances indicative of the human respiration tract, were observed in 3D-cultured human airway epithelial (HAE) cultures, but not in monolayer-cultured cells. Nucleic acid levels of HRV-C and HBoV and levels of virus-induced cytokines were also measured using the 3D culture system. Conclusion Our data provide a preliminary indication that the 3D culture model of primary epithelia using a Matrigel scaffold in vitro can be used to propagate HRV-C and HBoV. |
| WOS关键词 | RESPIRATORY SYNCYTIAL VIRUS ; HELPER TYPE-2 CYTOKINES ; LIQUID INTERFACE ; GENE-EXPRESSION ; CANCER-CELLS ; REPLICATION ; BRONCHIOLITIS ; HEART ; PROPAGATION ; STABILITY |
| 资助项目 | Major Project Specialized for Infectious Diseases of the Chinese Health and Family Planning Commission[2014ZX10004002-004-002] ; Major Project Specialized for Infectious Diseases of the Chinese Health and Family Planning Commission[2014ZX10004002-004-001] ; Young Talent Scholar Plan of Higher School in Hebei Province[BJ2017008] |
| WOS研究方向 | Environmental Sciences & Ecology ; Public, Environmental & Occupational Health |
| 语种 | 英语 |
| WOS记录号 | WOS:000427925500006 |
| 出版者 | CHINESE CENTER DISEASE CONTROL & PREVENTION |
| 资助机构 | Major Project Specialized for Infectious Diseases of the Chinese Health and Family Planning Commission ; Young Talent Scholar Plan of Higher School in Hebei Province |
| 源URL | [http://119.78.100.186/handle/113462/47419]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Hu Bu Rong |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Gansu, Peoples R China 2.Chinese Acad Sci, Key Lab Space Radiobiol Gansu Prov, Inst Modern Phys, Lanzhou 730000, Gansu, Peoples R China 3.Chengde Med Univ, Dept Pathogen Biol, Chengde 067000, Hebei, Peoples R China 4.Chinese Ctr Dis Control & Prevent, Natl Inst Viral Dis Control & Prevent, Dept Viral Diarrhea, Beijing 102206, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen Ya Xiong,Xie Guang Cheng,Pan Dong,et al. Three-dimensional Culture of Human Airway Epithelium in Matrigel for Evaluation of Human Rhinovirus C and Bocavirus Infections[J]. BIOMEDICAL AND ENVIRONMENTAL SCIENCES,2018,31:136-145. |
| APA | Chen Ya Xiong.,Xie Guang Cheng.,Pan Dong.,Du Ya Rong.,Pang Li Li.,...&Hu Bu Rong.(2018).Three-dimensional Culture of Human Airway Epithelium in Matrigel for Evaluation of Human Rhinovirus C and Bocavirus Infections.BIOMEDICAL AND ENVIRONMENTAL SCIENCES,31,136-145. |
| MLA | Chen Ya Xiong,et al."Three-dimensional Culture of Human Airway Epithelium in Matrigel for Evaluation of Human Rhinovirus C and Bocavirus Infections".BIOMEDICAL AND ENVIRONMENTAL SCIENCES 31(2018):136-145. |
入库方式： OAI收割
来源：近代物理研究所
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