Radiosensitization by Inhibiting Complex I activity in Human Hepatoma HepG2 cells to X-ray Radiation
文献类型：期刊论文
| 作者 | Zhang, Xin1,2,3 ; Zhou, Xin1,2,3; Chen, Ruping4; Zhang, Hong1,2,3
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| 刊名 | JOURNAL OF RADIATION RESEARCH
 |
| 出版日期 | 2012-03-01 |
| 卷号 | 53页码:257-263 |
| 关键词 | X-ray Radiation Radiosensitivity Mitochondrial Respiratory Chain Complex i Inhibition Reactive Oxygen Species |
| ISSN号 | 0449-3060 |
| DOI | 10.1269/jrr.11124 |
| 文献子类 | Article |
| 英文摘要 | The purpose of this study is to investigate the influence of mitochondrial respiratory chain complex I inhibition on the radiosensitivity of HepG2 cells. The complex I inhibitor rotenone was used to inhibit complex I activity on HepG2 cells before X-ray irradiation. The cytotoxicity of rotenone was analyzed by MTT assay at various doses. Rotenone induced dissipation of mitochondrial membrane potential and increase of intracellular ROS production were observed. Intracellular ATP production level was determined using luciferin-luciferase assay kit. We further analyzed cell survival and cell cycle distribution of a combined treatment which HepG2 cells underwent 0.5 mu M rotenone pretreatment firstly and irradiated with different doses of X-ray radiation afterwards. Our results suggest rotenone pretreatment prior to X-ray irradiation could induce a sensitizing effect on HepG2 cells by enhancing X-ray radiation induced proliferation inhibition and cell apoptosis. |
| WOS关键词 | HEPATOCELLULAR-CARCINOMA ; OXIDATIVE STRESS ; CANCER-CELLS ; APOPTOSIS ; ROS ; CYTOTOXICITY ; INVOLVEMENT ; SUPEROXIDE ; INDUCTION ; THERAPY |
| 资助项目 | National Basic Research Program of China (973 Program)[2010CB834202] |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics ; Radiology, Nuclear Medicine & Medical Imaging |
| 语种 | 英语 |
| WOS记录号 | WOS:000307196400013 |
| 出版者 | OXFORD UNIV PRESS |
| 源URL | [http://119.78.100.186/handle/113462/47778]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Zhang, Hong |
| 作者单位 | 1.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Peoples R China 3.Key Lab Heavy Ion Radiat Med Gansu Prov, Lanzhou 730000, Peoples R China 4.Lanzhou Univ, Sch Pharmaceut, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Xin,Zhou, Xin,Chen, Ruping,et al. Radiosensitization by Inhibiting Complex I activity in Human Hepatoma HepG2 cells to X-ray Radiation[J]. JOURNAL OF RADIATION RESEARCH,2012,53:257-263. |
| APA | Zhang, Xin,Zhou, Xin,Chen, Ruping,&Zhang, Hong.(2012).Radiosensitization by Inhibiting Complex I activity in Human Hepatoma HepG2 cells to X-ray Radiation.JOURNAL OF RADIATION RESEARCH,53,257-263. |
| MLA | Zhang, Xin,et al."Radiosensitization by Inhibiting Complex I activity in Human Hepatoma HepG2 cells to X-ray Radiation".JOURNAL OF RADIATION RESEARCH 53(2012):257-263. |
入库方式： OAI收割
来源：近代物理研究所
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