Protective effects of melatonin against C-12(6+) beam irradiation-induced oxidative stress and DNA injury in the mouse brain
文献类型：期刊论文
| 作者 | Wu, Z. H.1,2; Zhang, H.1 ; Wang, X. Y.2; Yang, R.3; Liu, B.2; Liu, Y.1; Zhao, W. P.1; Feng, H. Y.2; Xue, L. G.4; Hao, J. F.1
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| 刊名 | ADVANCES IN SPACE RESEARCH
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| 出版日期 | 2012 |
| 卷号 | 49页码:196-203 |
| 关键词 | Melatonin Radiation Brain Injury Carbon Ions Oxidative Stress |
| ISSN号 | 0273-1177 |
| DOI | 10.1016/j.asr.2011.09.007 |
| 文献子类 | Article |
| 英文摘要 | The purpose of this experiment was to estimate the protective effects of melatonin against radiation-induced brain damages in mice induced by heavy ion beams. Kun-Ming mice were randomly divided into five groups: normal control group, irradiation control group, and three different doses of melatonin (5, 10, and 20 mg/kg, i.p.) treated groups. Apart from the normal control group, the other four groups were exposed to whole-body 4.0 Gy carbon ion beam irradiation (approximately 0.5 Gy/min) after i.p. administration of normal saline or melatonin 1 h before irradiation. The oxidative redox status of brain tissue was assessed by measurement of malondiadehyde (MDA) levels, total superoxide dismutase (T-SOD), cytosolic superoxide dismutase (Cu/ZnSOD, SOD1) and mitochondrial superoxide dismutase (MnSOD, SOD2) activities at 8 h after irradiation. DNA damages were determined using the Comet assay and apoptosis and cell cycle distribution were detected by flow cytometric analyses. A dramatic dose-dependent decrease in MDA levels, tail moment, rates of tailing cells, and apoptosis, and a dose-dependent increase in T-SOD and SOD2 activities, in brain tissues in the melatonin-treated groups were detected compared with the irradiation only group. Furthermore, flow cytometric analysis demonstrated that the percentage of brain cells in the G0/G1 phase decreased significantly, while those in the S and G2/M stage increased dramatically, with mice pretreated with melatonin compared to the irradiation control group. These data indicate that melatonin has protective effects against irradiation-induced brain injury, and that its underlying protective mechanisms may relate to modulation of oxidative stress induced by heavy ionirradiation. (C) 2011 COSPAR. Published by Elsevier Ltd. All rights reserved. |
| WOS关键词 | PINEAL HORMONE MELATONIN ; TUMOR STEM-CELLS ; FREE-RADICALS ; VITAMIN-E ; IN-VIVO ; LIPID-PEROXIDATION ; IONIZING-RADIATION ; COMET ASSAY ; FEMALE RATS ; DAMAGE |
| 资助项目 | Natural Science Foundation of Gansu Province of China[0803RJZA074] |
| WOS研究方向 | Astronomy & Astrophysics ; Geology ; Meteorology & Atmospheric Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000299972200018 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://119.78.100.186/handle/113462/47793]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Zhang, H. |
| 作者单位 | 1.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China 2.Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Peoples R China 3.Tumour Hosp Gansu Prov, Pathol Diagnost Ctr, Lanzhou 730050, Peoples R China 4.Lanzhou Jiaotong Univ, Sch Chem & Biol Engn, Lanzhou 730070, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Z. H.,Zhang, H.,Wang, X. Y.,et al. Protective effects of melatonin against C-12(6+) beam irradiation-induced oxidative stress and DNA injury in the mouse brain[J]. ADVANCES IN SPACE RESEARCH,2012,49:196-203. |
| APA | Wu, Z. H..,Zhang, H..,Wang, X. Y..,Yang, R..,Liu, B..,...&Wang, Z. H..(2012).Protective effects of melatonin against C-12(6+) beam irradiation-induced oxidative stress and DNA injury in the mouse brain.ADVANCES IN SPACE RESEARCH,49,196-203. |
| MLA | Wu, Z. H.,et al."Protective effects of melatonin against C-12(6+) beam irradiation-induced oxidative stress and DNA injury in the mouse brain".ADVANCES IN SPACE RESEARCH 49(2012):196-203. |
入库方式： OAI收割
来源：近代物理研究所
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