Effects of carbon-ion beam or X-ray irradiation on anti-apoptosis Delta Np73 expression in HeLa cells
文献类型：期刊论文
| 作者 | Di, Cui-Xia1,3,4 ; Yang, Li-Na2; Zhang, Hong1,2,3; An, Li-Zhe2; Zhang, Xin1,2,3; Ma, Xiao-Fei1,2,3; Sun, Chao1,2,3; Wang, Xiao-Hu5; Yang, Rong6; Wu, Zhen-hua1,3,4
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| 刊名 | GENE
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| 出版日期 | 2013-02-15 |
| 卷号 | 515页码:208-213 |
| 关键词 | Carbon-ion Beam X-ray Apoptosis Cervical Carcinoma Delta Np73 |
| ISSN号 | 0378-1119 |
| DOI | 10.1016/j.gene.2012.11.040 |
| 文献子类 | Article |
| 英文摘要 | Delta Np73 has emerged as an interesting novel factor in cancer research. Here, we report the effect of carbon-ion beams on Delta Np73 expression in human cervix carcinoma HeLa cells in contrast to the effect of X-rays. Cellular sensitivities were determined by colony formation. Radiation-induced cell cycle arrest was investigated with flow cytometry. Additionally, radiation-induced apoptosis was analyzed with flow cytometry and Hoechst staining. Furthermore, Delta Np73 expression was examined by semi-quantitative reverse transcription-PCR (semi-quantitative RT-PCR) as well as by Western blot analysis. Following irradiation, stronger G2/M phase arrest, more significant increase in apoptosis and more pronounced Delta Np73 degradation were observed after exposure to high-LET carbon beams in comparison with X-rays at 4 Gy doses. These observations indicate that there is a differential Delta Np73 expression in response to different LET radiations, and down-regulated Delta Np73 expression might play a critical role in promoting cycle arrest and apoptosis in cancer cells. This study highlights the potential of Delta Np73 in radiotherapy. (C) 2012 Elsevier B.V. All rights reserved. |
| WOS关键词 | CERVICAL-CANCER CELLS ; HIGH-LET RADIATION ; IN-VIVO ; POOR-PROGNOSIS ; OVARIAN-CANCER ; LUNG-CANCER ; THERAPY ; P53 ; RADIOTHERAPY ; INDUCTION |
| 资助项目 | Western Talent Program of Chinese Academy of Sciences[Y260230XB0] |
| WOS研究方向 | Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000314559400030 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.186/handle/113462/48041]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Zhang, Hong |
| 作者单位 | 1.Chinese Acad Sci, Inst Modern Phys, Dept Heavy Ion Radiat Med, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Sch Life Sci, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Peoples R China 4.Key Lab Heavy Ion Radiat Med Gansu Prov, Lanzhou 730000, Peoples R China 5.Gansu Prov Canc Hosp, Dept Radiotherapy, Lanzhou 730050, Peoples R China 6.Gansu Prov Canc Hosp, Dept Pathol, Lanzhou 730050, Peoples R China |
| 推荐引用方式 GB/T 7714 | Di, Cui-Xia,Yang, Li-Na,Zhang, Hong,et al. Effects of carbon-ion beam or X-ray irradiation on anti-apoptosis Delta Np73 expression in HeLa cells[J]. GENE,2013,515:208-213. |
| APA | Di, Cui-Xia.,Yang, Li-Na.,Zhang, Hong.,An, Li-Zhe.,Zhang, Xin.,...&Si, Jing.(2013).Effects of carbon-ion beam or X-ray irradiation on anti-apoptosis Delta Np73 expression in HeLa cells.GENE,515,208-213. |
| MLA | Di, Cui-Xia,et al."Effects of carbon-ion beam or X-ray irradiation on anti-apoptosis Delta Np73 expression in HeLa cells".GENE 515(2013):208-213. |
入库方式： OAI收割
来源：近代物理研究所
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