Proteome analysis for profiling infertility markers in male mouse sperm after carbon ion radiation
文献类型：期刊论文
| 作者 | Li, Hong Yan1,2,3; Zhang, Hong1,2,3,4
|
| 刊名 | TOXICOLOGY
 |
| 出版日期 | 2013-04-05 |
| 卷号 | 306页码:85-92 |
| 关键词 | Proteome Sperm Carbon Ion radiatIon |
| ISSN号 | 0300-483X |
| DOI | 10.1016/j.tox.2013.02.008 |
| 文献子类 | Article |
| 英文摘要 | Ion radiation or radiotherapy is used to treat male patients with oligozoospermia, azoospermia, temporarily infertility, or even permanent infertility. The present study aims to investigate the potential infertility mechanism of sperm in mice after carbon ion radiation (CIR). The caudal epididymal sperm of male mice whole-body irradiated with carbon ion beam (0.5 Gy and 4 Gy) were used 7 days after irradiation. A two-dimensional gel electrophoresis approach was employed to investigate the changes in protein expression in the caudal edididymal sperm. Spot detection and matching were performed using the PDQuest 8.0 software. The criteria used to select spots for the analysis were more than a threefold difference in protein quantities (normalized spot volume), which allowed the detection of six differentially expressed proteins. Protein identification was performed using MALDI-TOF-TOF. Six specific proteins were identified by searching the NCBI protein sequence database. Among these proteins, HSP 70-2, PLC, GPX4, beta-tubulin, and GAPDHS were associated with sperm motility, which can affect fertility. beta-tubulin is important in axoneme migration flagellar movement and regulation, and GAPDHS is related to sperm energy supply. We analyzed their expressions using immunoblotting and immunofluorescence. The changes in sperm protein expression after CIR are mainly associated with motility. These proteins are potential markers for the mechanisms of infertility in space or radiotherapy. (C) 2013 Elsevier Ireland Ltd. All rights reserved. |
| WOS关键词 | HEAT-SHOCK PROTEINS ; SPERMATOZOA ; IRRADIATION ; EXPRESSION ; SPERMATOGENESIS ; TESTIS ; CELLS ; CAPACITATION ; LOCALIZATION ; APOPTOSIS |
| 资助项目 | Scientific Technology Research Projects of Gansu Province[0702NKDA045] ; Scientific Technology Research Projects of Gansu Province[0806RJYA020] |
| WOS研究方向 | Pharmacology & Pharmacy ; Toxicology |
| 语种 | 英语 |
| WOS记录号 | WOS:000318377500011 |
| 出版者 | ELSEVIER IRELAND LTD |
| 源URL | [http://119.78.100.186/handle/113462/48237]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Zhang, Hong |
| 作者单位 | 1.Chinese Acad Sci, Inst Modern Phys, Dept Heavy Ion Radiat Med, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Peoples R China 3.Key Lab Heavy Ion Radiat Med Gansu Prov, Lanzhou 730000, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Hong Yan,Zhang, Hong. Proteome analysis for profiling infertility markers in male mouse sperm after carbon ion radiation[J]. TOXICOLOGY,2013,306:85-92. |
| APA | Li, Hong Yan,&Zhang, Hong.(2013).Proteome analysis for profiling infertility markers in male mouse sperm after carbon ion radiation.TOXICOLOGY,306,85-92. |
| MLA | Li, Hong Yan,et al."Proteome analysis for profiling infertility markers in male mouse sperm after carbon ion radiation".TOXICOLOGY 306(2013):85-92. |
入库方式： OAI收割
来源：近代物理研究所
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