Effects of C-12(6+) ion radiation and ferulic acid on the zebrafish (Danio rerio) embryonic oxidative stress response and gene expression
文献类型：期刊论文
| 作者 | Si, Jing1,2,3 ; Zhang, Hong1,2,3; Wang, Zhenhua1,2,3; Wu, Zhenhua1,2,3; Lu, Jiang4; Di, Cuixia1,2,3; Zhou, Xin1,2,3; Wang, Xiaowei4
|
| 刊名 | MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS
 |
| 出版日期 | 2013-05-01 |
| 卷号 | 745页码:26-33 |
| 关键词 | C-12(6+) Ion Ferulic Acid Oxidative Stress Gene Expression |
| ISSN号 | 0027-5107 |
| DOI | 10.1016/j.mrfmmm.2013.03.007 |
| 文献子类 | Article |
| 英文摘要 | The effects of carbon ion irradiation and ferulic acid (FA) on the induction of oxidative stress and alteration of gene expression were studied in zebrafish (Danio rerio) embryos. Zebrafish embryos at 8 hpf were divided into seven groups: the control group; the 1 Gy, 3 Gy and 7 Gy irradiation groups; and three FA-pre-treated irradiation groups. In the irradiated groups, a significant increase in the teratogenesis of the zebrafish embryos and oxidative stress was accompanied by increased malondialdehyde (MDA) content, decreased glutathione (GSH) content and alterations in antioxidant enzyme activities (such as catalase [CAT] and superoxide dismutase [SOD]). Moreover, the mRNA levels for Cu/Zn-sod, Mn-sod, cat and gpx, the genes encoding these antioxidant proteins, were altered significantly. However, the mRNA expression patterns were not in accordance with those of the antioxidant enzymes and were more sensitive under low-dose irradiation. In addition, we detected the mRNA expression of ucp-2 and bcl-2, which are located at the mitochondrial inner membrane and related to reactive oxidative species (ROS) production. In the irradiated groups, the mRNA level of ucp-2 was significantly increased, whereas the mRNA level of bcl-2 was significantly decreased. Supplementation with FA, an antioxidant, was better able to reduce the irradiation-induced oxidative damage marked by changes in mortality, morphology, antioxidant enzyme activities and the MDA and GSH content, as well as in the mRNA expression levels. Overall, this study provided helpful information about the transcriptional effects of irradiation to better understand the mechanism of carbon ion-induced oxidative stress and FA-induced radioprotective effects. (C) 2013 Elsevier B.V. All rights reserved. |
| WOS关键词 | SALMON SALMO-SALAR ; TERM SPACE-FLIGHTS ; SWISS ALBINO MICE ; BLOOD-LYMPHOCYTES ; GAMMA-RADIATION ; DNA-DAMAGE ; CHROMOSOMAL-ABERRATIONS ; IONIZING-RADIATION ; LIPID-PEROXIDATION ; INDUCED APOPTOSIS |
| 资助项目 | Scientific Technology Research Projects of Gansu Province[0702NKDA045] ; Scientific Technology Research Projects of Gansu Province[0806RJYA020] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Genetics & Heredity ; Toxicology |
| 语种 | 英语 |
| WOS记录号 | WOS:000320828000004 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.186/handle/113462/48263]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Zhang, Hong |
| 作者单位 | 1.Chinese Acad Sci, Inst Modern Phys, Dept Heavy Ion Radiat Med, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Peoples R China 3.Key Lab Heavy Ion Radiat Med Gansu Prov, Lanzhou 730000, Peoples R China 4.Shihezi Univ, Coll Pharm, Key Lab Xinjiang Phytomed Resources, Shihezi 832002, Peoples R China |
| 推荐引用方式 GB/T 7714 | Si, Jing,Zhang, Hong,Wang, Zhenhua,et al. Effects of C-12(6+) ion radiation and ferulic acid on the zebrafish (Danio rerio) embryonic oxidative stress response and gene expression[J]. MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS,2013,745:26-33. |
| APA | Si, Jing.,Zhang, Hong.,Wang, Zhenhua.,Wu, Zhenhua.,Lu, Jiang.,...&Wang, Xiaowei.(2013).Effects of C-12(6+) ion radiation and ferulic acid on the zebrafish (Danio rerio) embryonic oxidative stress response and gene expression.MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS,745,26-33. |
| MLA | Si, Jing,et al."Effects of C-12(6+) ion radiation and ferulic acid on the zebrafish (Danio rerio) embryonic oxidative stress response and gene expression".MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS 745(2013):26-33. |
入库方式： OAI收割
来源：近代物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。