Differential Proteomic Analysis of Carbon Ion Radiation in Sheep Sperm
文献类型：期刊论文
| 作者 | He Yu-xuan1; Li Hong-yan2,3,4,5; Zhang Yong1; He Jian-hua6; Zhang Hong2,3,4 ; Zhao Xing-xu1
|
| 刊名 | JOURNAL OF INTEGRATIVE AGRICULTURE
 |
| 出版日期 | 2013 |
| 卷号 | 12页码:1629-1637 |
| 关键词 | Sheep Sperm Protein Two-dimensional Polyacrylamide Gel Electrophoresis Analysis Proteome Carbon Ion radiatIon Irradiation Breeding |
| ISSN号 | 2095-3119 |
| DOI | 10.1016/S2095-3119(13)60558-4 |
| 文献子类 | Article |
| 英文摘要 | This study is first to investigate proteomic changes in sheep sperm induced by carbon ion radiation using two-dimensional electrophoresis (2-DE) analysis in the project of breeding a new variety of sheep. Differential expression proteins were detected using the PDQuest 8.0 software after staining with Coomassie blue. Valid spots were then analyzed through liquid chromatography tandem mass spectrometry (LC-MS/MS). Among the 480 total protein spots displayed in 2-D gels, 6 specific protein spots were observed in sperm gels. A search against protein sequences in the National Center for Biotechnology Information databases (NCBI) indicated that differentially expressed proteins correspond to two proteins, identified to be enolase and transcription factor AP-2-alpha (TFAP-2 alpha). The two proteins were up-regulated in the irradiated sperm. To the best of our knowledge, this study is the first to identify proteomic changes induced by carbon ion radiation in sheep sperm. The analysis of differential expression protein may be useful in identifying new breeding markets in sheep reproduction and in clarifying the mechanisms involved in irradiation or space breeding. |
| WOS关键词 | TRANSCRIPTION FACTOR AP-2-ALPHA ; PROTEINS ; APOPTOSIS ; IRRADIATION ; BINDING ; ENOLASE ; CELLS |
| 资助项目 | National Natural Science Foundation of China[2010CB834202] |
| WOS研究方向 | Agriculture |
| 语种 | 英语 |
| WOS记录号 | WOS:000324348900015 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://119.78.100.186/handle/113462/48289]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Zhao Xing-xu |
| 作者单位 | 1.Gansu Agr Univ, Coll Vet Med, Lanzhou 730070, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Dept Med Phys, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Key Lab Heavy Ion Radiat Med, Lanzhou 730000, Peoples R China 4.Key Lab Heavy Ion Radiat Med Gansu Prov, Lanzhou 730000, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.Lanzhou Agr Sci & Technol Res Promot Ctr, Lanzhou 730030, Peoples R China |
| 推荐引用方式 GB/T 7714 | He Yu-xuan,Li Hong-yan,Zhang Yong,et al. Differential Proteomic Analysis of Carbon Ion Radiation in Sheep Sperm[J]. JOURNAL OF INTEGRATIVE AGRICULTURE,2013,12:1629-1637. |
| APA | He Yu-xuan,Li Hong-yan,Zhang Yong,He Jian-hua,Zhang Hong,&Zhao Xing-xu.(2013).Differential Proteomic Analysis of Carbon Ion Radiation in Sheep Sperm.JOURNAL OF INTEGRATIVE AGRICULTURE,12,1629-1637. |
| MLA | He Yu-xuan,et al."Differential Proteomic Analysis of Carbon Ion Radiation in Sheep Sperm".JOURNAL OF INTEGRATIVE AGRICULTURE 12(2013):1629-1637. |
入库方式： OAI收割
来源：近代物理研究所
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