中国科学院机构知识库网格系统: Chromosome aberrations in normal human fibroblasts analyzed in G(0)/G(1) and G(2)/M phases after exposure in G(0) to radiation with different linear energy transfer (LET)

Chromosome aberrations in normal human fibroblasts analyzed in G(0)/G(1) and G(2)/M phases after exposure in G(0) to radiation with different linear energy transfer (LET)

文献类型：期刊论文


作者	Liu, Cuihua 1; Kawata, Tetsuya 2; Furusawa, Yoshiya 1; Zhou, Guangming4 ; Inoue, Kohei 5; Fukada, Junichi 2; Kota, Ryuichi 2; George, Kerry 6; Cucinotta, Francis 7; Okayasu, Ryuichi 3
刊名	MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
出版日期	2013-08-30
卷号	756 页码:101-107
ISSN号	1383-5718
关键词	Chromosome Aberration Premature Chromosome Condensation (Pcc) Fluorescence In Situ Hybridization (Fish) High-let Radiation Cell Cycle Arrest
DOI	10.1016/j.mrgentox.2013.05.005
文献子类	Article
英文摘要	We have studied the induction of chromosome aberrations in human fibroblasts exposed in G(0)/G(1) to X-rays or heavy ions to study the influence of G(1) cell cycle arrest. Confluent normal fibroblasts were exposed to X-rays or accelerated particles with different LET values and chromosome aberrations were investigated in the first G(0)/G(1) and G(2)//M phase. The particles used here were 490 MeV/nucleon Si, 500 MeV/nucleon Fe, and 200 MeV/nucleon Fe ions. Cells were subcultured 24 h after exposure and premature chromosome condensation (PCC) was performed by fusion-induced method for analysis of G(0)/G(1) cells, and chemically-induced method for analysis of G(2) and metaphase cells. Chromosome damage was assessed in chromosomes 1 and 3 using whole chromosome fluorescence in situ hybridization (FISH). Cell cycle was analyzed by flow cytometry at different incubation times following subculture. After irradiation with 2 Gy of high-LET particles, the yields of chromosome aberrations and fragments were significantly higher in G(0)/G(1) phase than in G(2)/M phase, whereas similar yields of damage were measured in both phases after exposure to X-rays. In contrast, the yield of misrepair, assessed by the number of color junctions, was similar in the G(0)/G(1) and G(2)/M phases after exposure to either X-rays or high-LET particles. The yields of chromosome aberrations, fragments, and color junctions in both the G(0)/G(1) and the G(2)/M phases, increased with LET up to 200 keV/mu m, then decreased for 440 key/mu m Fe particles. A good correlation was found between chromosome aberrations in both G(0)/G(1) and G(2)/M cells and survival fractions after 2 Gy of different LET radiations, although the slopes were steeper for the G(0)/G(1) cells. Flow cytometry analysis indicated that high-LET particles induce more non cycling G(0)/G(1) cells within 48 h of subculture than X-rays, suggesting that chromosome aberrations scored at the G(2)/M phase may not accurately describe the true radiation effect. (C) 2013 Elsevier B.V. All rights reserved.
WOS关键词	POTENTIALLY LETHAL DAMAGE ; IN-SITU HYBRIDIZATION ; DOUBLE-STRAND BREAKS ; CELL-CYCLE ARREST ; HUMAN-LYMPHOCYTES ; X-RAYS ; EXCHANGE FORMATION ; TIME-COURSE ; FE-IONS ; C-IONS
资助项目	Japan Society for the Promotion of Science (JSPS) KAKENHI[24591859] ; Japan Society for the Promotion of Science (JSPS) KAKENHI[24249067] ; Japan Society for the Promotion of Science (JSPS) KAKENHI[23591849]
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity ; Toxicology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000325600300014
源URL	[http://119.78.100.186/handle/113462/48335]
专题	中国科学院近代物理研究所
通讯作者	Kawata, Tetsuya
作者单位	1.Natl Inst Radiol Sci, Res Ctr Charged Particle Therapy, Chiba 2638555, Japan 2.Keio Univ, Sch Med, Dept Radiol, Tokyo 1608582, Japan 3.Natl Inst Radiol Sci, Int Open Lab, Chiba 2638555, Japan 4.Chinese Acad Sci, Inst Modern Phys, Dept Space Radiobiol, Lanzhou 730000, Peoples R China 5.Chiba Univ, Grad Sch Med, Dept Radiol, Chiba 2608670, Japan 6.Wyle Sci, Technol & Engn Grp, Houston, TX 77058 USA 7.NASA, Lyndon B Johnson Space Ctr, Houston, TX 77058 USA
推荐引用方式 GB/T 7714	Liu, Cuihua,Kawata, Tetsuya,Furusawa, Yoshiya,et al. Chromosome aberrations in normal human fibroblasts analyzed in G(0)/G(1) and G(2)/M phases after exposure in G(0) to radiation with different linear energy transfer (LET)[J]. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS,2013,756:101-107.
APA	Liu, Cuihua.,Kawata, Tetsuya.,Furusawa, Yoshiya.,Zhou, Guangming.,Inoue, Kohei.,...&Okayasu, Ryuichi.(2013).Chromosome aberrations in normal human fibroblasts analyzed in G(0)/G(1) and G(2)/M phases after exposure in G(0) to radiation with different linear energy transfer (LET).MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS,756,101-107.
MLA	Liu, Cuihua,et al."Chromosome aberrations in normal human fibroblasts analyzed in G(0)/G(1) and G(2)/M phases after exposure in G(0) to radiation with different linear energy transfer (LET)".MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS 756(2013):101-107.

入库方式： OAI收割

来源：近代物理研究所

下载0

Chromosome aberrations in normal human fibroblasts analyzed in G(0)/G(1) and G(2)/M phases after exposure in G(0) to radiation with different linear energy transfer (LET)

其他版本