Biological characteristics of Rh123(high) stem-like cells in a side population of 786-O renal carcinoma cells
文献类型：期刊论文
| 作者 | Lu, Jianzhong1; Cui, Yong1; Zhu, Jiayun2 ; He, Jinpeng2; Zhou, Guangming2; Yue, Zhongjin1
|
| 刊名 | ONCOLOGY LETTERS
 |
| 出版日期 | 2013-06-01 |
| 卷号 | 5页码:1903-1908 |
| 关键词 | Rhodamine 123 Cancer Stem Cell Renal Cell Carcinoma Flow Cytometery Tumorigenesis |
| ISSN号 | 1792-1074 |
| DOI | 10.3892/ol.2013.1270 |
| 文献子类 | Article |
| 英文摘要 | The aim of the present study was to investigate the differences in biological characteristics between the rhodamine 123 (Rh123)(high) and Rh123(low) subpopulations of the renal cancer cell line 786-O and to identify evidence for the existence of cancer stem cells in renal cell carcinoma (RCC) cells. In vitro cultured RCC 786-O cells were stained with Rh123, analyzed and sorted with flow cytometry. The differences in proliferative activity, long-term differentiation and radiation sensitivity between the two subpopulations were measured and the oncogenicity of each subpopulation was evaluated according to their neoplastic growth ability in soft agar and tumor-forming ability in NOD/SCID immunodeficient mice. There were two subpopulations in the cultured 786-O cells, Rh123(high) and Rh123(low) cells. Rh123(low) cells were the majority among 786-O renal carcinoma cells and barely formed solid tumors in NOD/SCID mice and colonies in soft agar. By contrast, the Rh123(high) cells were the minority, exhibited high proliferative activity, differentiation ability and resistance to radiation and showed high tumorigenesis potential and colony forming efficiency. The Rh123(high) cells had stem-like characteristics in cultured RCC 786-O cells in vitro. |
| WOS关键词 | PROSPECTIVE IDENTIFICATION |
| 资助项目 | National Natural Science Foundation of China[30571860] |
| WOS研究方向 | Oncology |
| 语种 | 英语 |
| WOS记录号 | WOS:000324816500029 |
| 出版者 | SPANDIDOS PUBL LTD |
| 源URL | [http://119.78.100.186/handle/113462/48351]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Yue, Zhongjin |
| 作者单位 | 1.Lanzhou Univ, Hosp 2, Key Lab Urol Dis Gansu Prov, Inst Urol,Gansu Nephrourol Clin Ctr, Lanzhou 730030, Gansu, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Radiobiol Effects Grp, Lanzhou 730000, Gansu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu, Jianzhong,Cui, Yong,Zhu, Jiayun,et al. Biological characteristics of Rh123(high) stem-like cells in a side population of 786-O renal carcinoma cells[J]. ONCOLOGY LETTERS,2013,5:1903-1908. |
| APA | Lu, Jianzhong,Cui, Yong,Zhu, Jiayun,He, Jinpeng,Zhou, Guangming,&Yue, Zhongjin.(2013).Biological characteristics of Rh123(high) stem-like cells in a side population of 786-O renal carcinoma cells.ONCOLOGY LETTERS,5,1903-1908. |
| MLA | Lu, Jianzhong,et al."Biological characteristics of Rh123(high) stem-like cells in a side population of 786-O renal carcinoma cells".ONCOLOGY LETTERS 5(2013):1903-1908. |
入库方式： OAI收割
来源：近代物理研究所
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