Comparison of the repair of potentially lethal damage after low- and high-LET radiation exposure, assessed from the kinetics and fidelity of chromosome rejoining in normal human fibroblasts
文献类型：期刊论文
| 作者 | Liu, Cuihua1; Kawata, Tetsuya2; Zhou, Guangming3 ; Furusawa, Yoshiya1; Kota, Ryuichi2; Kumabe, Atsuhiro2; Sutani, Shinya2; Fukada, Junichi2; Mishima, Masayo2; Shigematsu, Naoyuki2
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| 刊名 | JOURNAL OF RADIATION RESEARCH
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| 出版日期 | 2013-11-01 |
| 卷号 | 54页码:989-997 |
| 关键词 | Heavy Ion Pldr (Potentially Lethal Damage Repair) Premature Chromosome Condensation Fish (Fluorescence In Situ Hybridization) Misrepair |
| ISSN号 | 0449-3060 |
| DOI | 10.1093/jrr/rrt031 |
| 文献子类 | Article |
| 英文摘要 | Potentially lethal damage (PLD) and its repair (PLDR) were studied in confluent human fibroblasts by analyzing the kinetics of chromosome break rejoining after X-ray or heavy-ion exposures. Cells were either held in the non-cycling G(0) phase of the cell cycle for 12 h, or forced to proliferate immediately after irradiation. Fusion premature chromosome condensation (PCC) was combined with fluorescence in situ hybridization (FISH) to study chromosomal aberrations in interphase. The culture condition had no impact on the rejoining kinetics of PCC breaks during the 12 h after X-ray or heavy-ion irradiation. However, 12 h after X-ray and silicon irradiation, cycling cells had more chromosome exchanges than non-cycling cells. After 6 Gy X-rays, the yield of exchanges in cycling cells was 2.8 times higher than that in non-cycling cells, and after 2 Gy of 55 keV/mu m silicon ions the yield of exchanges in cycling cells was twice that of non-cycling cells. In contrast, after exposure to 2 Gy 200-keV/mu m or 440-keV/mu m iron ions the yield of exchanges was similar in non-cycling and cycling cells. Since the majority of repair in G(0)/G(1) occurs via the non-homologous end joining process (NHEJ), increased PLDR in X-ray and silicon-ion irradiated cells may result from improved cell cycle-specific rejoining fidelity through the NHEJ pathway, which is not the case in high-LET iron-ion irradiated cells. |
| WOS关键词 | IRRADIATED HUMAN-CELLS ; IN-SITU HYBRIDIZATION ; X-RAY ; EXCHANGE FORMATION ; ENHANCED FIDELITY ; HUMAN-LYMPHOCYTES ; DNA-DAMAGE ; HEAVY-IONS ; BREAKS ; CONDENSATION |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics ; Radiology, Nuclear Medicine & Medical Imaging |
| 语种 | 英语 |
| WOS记录号 | WOS:000327458900002 |
| 出版者 | OXFORD UNIV PRESS |
| 源URL | [http://119.78.100.186/handle/113462/48417]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Kawata, Tetsuya |
| 作者单位 | 1.Natl Inst Radiol Sci, Res Ctr Charged Particle Therapy, Chiba 2638555, Japan 2.Keio Univ, Sch Med, Dept Radiol, Tokyo 1608582, Japan 3.Chinese Acad Sci, Inst Modern Phys, Dept Space Radiobiol, Lanzhou 730000, Peoples R China 4.Wyle Integrated Sci & Engn Grp, Houston, TX USA 5.NASA, Lyndon B Johnson Space Ctr, Houston, TX 77058 USA |
| 推荐引用方式 GB/T 7714 | Liu, Cuihua,Kawata, Tetsuya,Zhou, Guangming,et al. Comparison of the repair of potentially lethal damage after low- and high-LET radiation exposure, assessed from the kinetics and fidelity of chromosome rejoining in normal human fibroblasts[J]. JOURNAL OF RADIATION RESEARCH,2013,54:989-997. |
| APA | Liu, Cuihua.,Kawata, Tetsuya.,Zhou, Guangming.,Furusawa, Yoshiya.,Kota, Ryuichi.,...&Cucinotta, Francis.(2013).Comparison of the repair of potentially lethal damage after low- and high-LET radiation exposure, assessed from the kinetics and fidelity of chromosome rejoining in normal human fibroblasts.JOURNAL OF RADIATION RESEARCH,54,989-997. |
| MLA | Liu, Cuihua,et al."Comparison of the repair of potentially lethal damage after low- and high-LET radiation exposure, assessed from the kinetics and fidelity of chromosome rejoining in normal human fibroblasts".JOURNAL OF RADIATION RESEARCH 54(2013):989-997. |
入库方式： OAI收割
来源：近代物理研究所
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