Mechanism of apoptotosis induced by ortho-topolin riboside in human hepatoma cell line SMMC-7721
文献类型：期刊论文
| 作者 | Wang, Li1; Sun, Chao2,3; Wang, Zhen-Hua2 ; Guo, Guang-Qin1
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| 刊名 | FOOD AND CHEMICAL TOXICOLOGY
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| 出版日期 | 2012-06-01 |
| 卷号 | 50页码:1962-1968 |
| 关键词 | Ortho-topolin Riboside Apoptosis Akt Erk1/2 Adenosine Kinase Human Hepatoma Cell |
| ISSN号 | 0278-6915 |
| DOI | 10.1016/j.fct.2012.03.053 |
| 文献子类 | Article |
| 英文摘要 | The naturally occurring cytokinin, ortho-topolin riboside (oTR), has been recently reported to have a strong anticancer effect. However, the molecular mechanism has not been elucidated. From our research we found that oTR strongly inhibited the proliferation of SMMC-7721 cells inducing apoptosis. After oTR treatment, up-regulation of the protein levels of pro-apoptotic Bax and the down-regulation of the antiapoptotic proteins, Bcl-2 and Bcl-xL was observed, leading to the loss of mitochondrial membrane potential, the release of cytochrome c from the mitochondria into the cytosol, the downstream activation of caspase-9 and caspase-3, as well as the cleavage of poly ADP-ribose-polymerase (PARP), the effect of apoptosis could be blocked by the pan-specific caspase inhibitor z-VAD-fmk and caspase-9-specific inhibitor z-LEHD-fmk. Moreover, oTR was shown to inhibit the activation of the extracellular signal-regulated kinase-1/2 (ERK1/2) as well as the Akt pathway. These results suggest that oTR interferes with the mitogen-activated protein kinase (MAPK) and Akt pathways and induces the apoptosis of human SMMC-7721 cells through the activation of intrinsic mitochondria-mediated pathways. However, the apoptosis was completely prevented when cells were treated with A-134974, an inhibitor of adenosine kinase, it indicated that the intracellular phosphorylation of oTR is necessary for its cytotoxic effects to SMMC-7721 cells. (C) 2012 Elsevier Ltd. All rights reserved. |
| WOS关键词 | ACTIVATED PROTEIN-KINASES ; MYELOID-LEUKEMIA CELLS ; BCL-2 FAMILY PROTEINS ; TOBACCO BY-2 CELLS ; HL-60 CELLS ; CANCER-CELLS ; KINETIN RIBOSIDE ; CORRESPONDING MONONUCLEOTIDES ; INDUCED DIFFERENTIATION ; CYTOKININ NUCLEOSIDES |
| 资助项目 | National Natural Science Foundation of China[31030045] |
| WOS研究方向 | Food Science & Technology ; Toxicology |
| 语种 | 英语 |
| WOS记录号 | WOS:000305719700022 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 源URL | [http://119.78.100.186/handle/113462/48979]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Guo, Guang-Qin |
| 作者单位 | 1.Lanzhou Univ, Dept Cell Biol, Sch Life Sci, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Li,Sun, Chao,Wang, Zhen-Hua,et al. Mechanism of apoptotosis induced by ortho-topolin riboside in human hepatoma cell line SMMC-7721[J]. FOOD AND CHEMICAL TOXICOLOGY,2012,50:1962-1968. |
| APA | Wang, Li,Sun, Chao,Wang, Zhen-Hua,&Guo, Guang-Qin.(2012).Mechanism of apoptotosis induced by ortho-topolin riboside in human hepatoma cell line SMMC-7721.FOOD AND CHEMICAL TOXICOLOGY,50,1962-1968. |
| MLA | Wang, Li,et al."Mechanism of apoptotosis induced by ortho-topolin riboside in human hepatoma cell line SMMC-7721".FOOD AND CHEMICAL TOXICOLOGY 50(2012):1962-1968. |
入库方式： OAI收割
来源：近代物理研究所
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