Heavy ion and X-ray irradiation alter the cytoskeleton and cytomechanics of cortical neurons
文献类型：期刊论文
| 作者 | Du, Yuting1,2; Zhang, Jie1,2; Zheng, Qian1; Li, Mingxin1; Liu, Yang3; Zhang, Baoping4; Liu, Bin1,2; Zhang, Hong3; Miao, Guoying5 |
| 刊名 | NEURAL REGENERATION RESEARCH
 |
| 出版日期 | 2014-06-01 |
| 卷号 | 9页码:1129-1137 |
| 关键词 | Nerve Regeneration Radiation Brain Injury Neurons Heavy Ion X-ray Cytoskeleton Cytomechanical Properties Atomic Force Microscopy Neural Regeneration |
| ISSN号 | 1673-5374 |
| DOI | 10.4103/1673-5374.135315 |
| 文献子类 | Article |
| 英文摘要 | Heavy ion beams with high linear energy transfer exhibit more beneficial physical and biological performance than conventional X-rays, thus improving the potential of this type of radiotherapy in the treatment of cancer. However, these two radiotherapy modalities both cause inevitable brain injury. The objective of this study was to evaluate the effects of heavy ion and X-ray irradiation on the cytoskeleton and cytomechanical properties of rat cortical neurons, as well as to determine the potential mechanism of neuronal injury after irradiation. Cortical neurons from 30 new-born mice were irradiated with heavy ion beams at a single dose of 2 Gy and X-rays at a single dose of 4 Gy; subsequent evaluation of their effects were carried out at 24 hours after irradiation. An immunofluorescence assay showed that after irradiation with both the heavy ion beam and X-rays, the number of primary neurons was significantly decreased, and there was evidence of apoptosis. Radiation-induced neuronal injury was more apparent after X-irradiation. Under atomic force microscopy, the neuronal membrane appeared rough and neuronal rigidity had increased. These cell changes were more apparent following exposure to X-rays. Our findings indicated that damage caused by heavy ion and X-ray irradiation resulted in the structural distortion and rearrangement of the cytoskeleton, and affected the cytomechanical properties of the cortical neurons. Moreover, this radiation injury to normal neurons was much severer after irradiation with X-rays than after heavy ion beam irradiation. |
| WOS关键词 | ATOMIC-FORCE MICROSCOPY ; INDUCED APOPTOSIS ; NERVOUS-SYSTEM ; BRAIN ; CELLS ; DYNAMICS ; AFM ; REGENERATION ; BIOMECHANICS ; ELASTICITY |
| WOS研究方向 | Cell Biology ; Neurosciences & Neurology |
| 语种 | 英语 |
| WOS记录号 | WOS:000338803900010 |
| 出版者 | SHENYANG EDITORIAL DEPT NEURAL REGENERATION RES |
| 源URL | [http://119.78.100.186/handle/113462/49893]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Liu, Bin |
| 作者单位 | 1.Lanzhou Univ, Sch Stomatol, Lanzhou 730000, Gansu, Peoples R China 2.Lanzhou Univ, Sch Nucl Sci & Technol, Lanzhou 730000, Gansu, Peoples R China 3.Chinese Acad Sci, Dept Radiat Biol & Med, Inst Modern Phys, Lanzhou, Gansu, Peoples R China 4.Lanzhou Univ, Sch Civil Engn & Mech, Lanzhou 730000, Gansu, Peoples R China 5.Gansu Prov Hosp, Lanzhou, Gansu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Du, Yuting,Zhang, Jie,Zheng, Qian,et al. Heavy ion and X-ray irradiation alter the cytoskeleton and cytomechanics of cortical neurons[J]. NEURAL REGENERATION RESEARCH,2014,9:1129-1137. |
| APA | Du, Yuting.,Zhang, Jie.,Zheng, Qian.,Li, Mingxin.,Liu, Yang.,...&Miao, Guoying.(2014).Heavy ion and X-ray irradiation alter the cytoskeleton and cytomechanics of cortical neurons.NEURAL REGENERATION RESEARCH,9,1129-1137. |
| MLA | Du, Yuting,et al."Heavy ion and X-ray irradiation alter the cytoskeleton and cytomechanics of cortical neurons".NEURAL REGENERATION RESEARCH 9(2014):1129-1137. |
入库方式： OAI收割
来源：近代物理研究所
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