Heavy ion radiation can promote greater motility and enolase protein expression in ram sperm in in vitro liquid storage
文献类型：期刊论文
| 作者 | He, Yuxuan1; Li, Hongyan2,3,4; He, Jianhua5; Zhao, Xingxu1 |
| 刊名 | ANIMAL REPRODUCTION SCIENCE
 |
| 出版日期 | 2014-08-01 |
| 卷号 | 148页码:260-266 |
| 关键词 | Ram Sperm Motility Heavy Ion radiatIon |
| ISSN号 | 0378-4320 |
| DOI | 10.1016/j.anireprosci.2014.06.019 |
| 文献子类 | Article |
| 英文摘要 | The aim of the study was to determine the effects of heavy ion radiation (HIR) on ram sperm quality during 24 h of in vitro liquid storage at 15 degrees C, and identify the most appropriate dose which did not injure, but actually improved sperm quality and confirmed the relationship between highly expressed enolase and ram sperm quality during storage in vitro. Six Dorset ram (Ovis aries) semen pools from five mature and healthy rams were each divided into seven experimental groups with different doses of HIR (0, 0.05, 0.1, 0.2, 0.3, 0.4, and 0.5 Gy) under the same experimental conditions. Sperm motility, viability, ATP content, and the gene and protein expression of enolase were measured at 24 h of storage. Irradiated semen which had been stored for 24h. retained not only greater sperm motility, viability, and ATP content, but had greater enolase protein expression. This was evidenced by increased amounts of mRNA for this enzyme and amount of enolase protein as compared with semen from control rams, especially for the 0.1 Gy group (P<0.001). These results indicate that HIR can promote enhanced motility and viability during in vitro liquid storage, and the 0.1 Gy may be a suitable dose for improving sperm quality. Greater amounts of enolase and ATP content may results from enhanced sperm glycolysis by HIR. HIR enhances sperm glycolysis to generate sufficient ATP for maintaining sperm motility during storage. (C) 2014 Elsevier B.V. All rights reserved. |
| WOS关键词 | PROTEOMIC ANALYSIS ; MOUSE SPERM ; X-RAY ; INFERTILITY ; GLYCOLYSIS ; DAMAGE ; CELLS ; RATS |
| 语种 | 英语 |
| WOS记录号 | WOS:000340221700021 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.186/handle/113462/49951]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Zhao, Xingxu |
| 作者单位 | 1.Gansu Agr Univ, Coll Vet Med, Lanzhou 730070, Peoples R China 2.Chinese Acad Sci, Inst Modem Phys, Dept Heavy Ion Radiat Med, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Peoples R China 4.Key Lab Heavy Ion Radiat Med Gansu Prov, Lanzhou 730000, Peoples R China 5.Lanzhou Agr Sci & Technol Res Promot Ctr, Lanzhou 730030, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, Yuxuan,Li, Hongyan,He, Jianhua,et al. Heavy ion radiation can promote greater motility and enolase protein expression in ram sperm in in vitro liquid storage[J]. ANIMAL REPRODUCTION SCIENCE,2014,148:260-266. |
| APA | He, Yuxuan,Li, Hongyan,He, Jianhua,&Zhao, Xingxu.(2014).Heavy ion radiation can promote greater motility and enolase protein expression in ram sperm in in vitro liquid storage.ANIMAL REPRODUCTION SCIENCE,148,260-266. |
| MLA | He, Yuxuan,et al."Heavy ion radiation can promote greater motility and enolase protein expression in ram sperm in in vitro liquid storage".ANIMAL REPRODUCTION SCIENCE 148(2014):260-266. |
入库方式： OAI收割
来源：近代物理研究所
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