Comparative Profiling of MicroRNAs Reveals the Underlying Toxicological Mechanism in Mice Testis Following Carbon Ion Radiation
文献类型：期刊论文
| 作者 | He, Yuxuan1; Zhang, Yong1 ; Li, Hongyan2; Zhang, Hong2; Li, Zongshuai1; Xiao, Longfei1; Hu, Junjie1; Ma, Youji1; Zhang, Quanwei1; Zhao, Xingxu1
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| 刊名 | DOSE-RESPONSE
 |
| 出版日期 | 2018-06-20 |
| 卷号 | 16页码:1559325818778633 |
| 关键词 | heavy ion radiation miRNAs testis |
| ISSN号 | 1559-3258 |
| DOI | 10.1177/1559325818778633 |
| 英文摘要 | This study investigated the toxicity of heavy ion radiation to mice testis by microRNA (miRNA) sequencing and bioinformatics analyses. Testicular indices and histology were measured following enterocoelia irradiation with a 2 Gy carbon ion beam, with the testes exhibiting the most serious injuries at 4 weeks after carbon ion radiation (CIR) exposure. Illumina sequencing technology was used to sequence small RNA libraries of the control and irradiated groups at 4 weeks after CIR. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses implicated differential miRNAs in the regulation of target genes involved in metabolism, development, and reproduction. Here, 8 miRNAs, including miR-34c-5p, miR-138, and 6 let-7 miRNA family members previously reported in testis after radiation, were analyzed by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) to validate miRNA sequencing data. The differentially expressed miRNAs described here provided a novel perspective for the role of miRNAs in testis toxicity following CIR. |
| WOS关键词 | UP-REGULATION ; SPERMATOGONIAL DIFFERENTIATION ; EXPRESSION PROFILES ; MALE-INFERTILITY ; CANCER-PATIENTS ; CELL APOPTOSIS ; SPERMATOGENESIS ; IRRADIATION ; SPERM ; ONCOFERTILITY |
| 资助项目 | Ministry of Science and Technology National Key RD project[2016YFC0904600] |
| WOS研究方向 | Pharmacology & Pharmacy ; Radiology, Nuclear Medicine & Medical Imaging ; Toxicology |
| 语种 | 英语 |
| WOS记录号 | WOS:000435970700001 |
| 出版者 | SAGE PUBLICATIONS INC |
| 源URL | [http://119.78.100.186/handle/113462/56857]  |
| 专题 | 中国科学院近代物理研究所 |
| 作者单位 | 1.Gansu Agr Univ, Coll Vet Med, Lanzhou 730070, Gansu, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Dept Radiat Med, Lanzhou, Gansu, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, Yuxuan,Zhang, Yong,Li, Hongyan,et al. Comparative Profiling of MicroRNAs Reveals the Underlying Toxicological Mechanism in Mice Testis Following Carbon Ion Radiation[J]. DOSE-RESPONSE,2018,16:1559325818778633. |
| APA | He, Yuxuan.,Zhang, Yong.,Li, Hongyan.,Zhang, Hong.,Li, Zongshuai.,...&Zhao, Xingxu.(2018).Comparative Profiling of MicroRNAs Reveals the Underlying Toxicological Mechanism in Mice Testis Following Carbon Ion Radiation.DOSE-RESPONSE,16,1559325818778633. |
| MLA | He, Yuxuan,et al."Comparative Profiling of MicroRNAs Reveals the Underlying Toxicological Mechanism in Mice Testis Following Carbon Ion Radiation".DOSE-RESPONSE 16(2018):1559325818778633. |
入库方式： OAI收割
来源：近代物理研究所
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