中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Potential mechanisms involved in resistant phenotype of MCF-7 breast carcinoma cells to ionizing radiation induced apoptosis

文献类型:会议论文

作者Wang, Yan-ling2,3,4; Zhang, Hong2,3; Li, Ning2,3,4; Wang, Xiao-hu1; Hao, Ji-fang2,3; Zhao, Wei-ping2,3,4
出版日期2009-03
关键词BRCA1 Phosphorylation Ionizing radiation (IR) p21 Bax Bcl-2
卷号267
期号6
DOI10.1016/j.nimb.2009.02.014
页码1001-1006
英文摘要In the present study, we investigated the mechanisms of apoptosis resistance and the roles of the phosphorylation of BRCA1, p21, the Bax/Bcl-2 protein ratio and cell cycle arrest in IR-induced apoptosis in MCF-7 cells. X-irradiation, in particular at low dose (1 Gy), but not carbon ion irradiation, had a significant anti proliferative effect on the growth of MCF-7 cells. I Gy X-irradiation resulted in G1 and G2 phase arrest, but 4 Gy induced a significant G1 block. In contrast, carbon ion irradiation resulted in a significant accumulation in the G2 phase. Concomitant with the phosphorylation of H2AX induced by DNA damage, carbon ion irradiation resulted in all approximately 1.9-2.8-fold increase in the phosphorylation of BRCA1 on serine residue 1524, significantly greater than that detected for X-irradiation. Carbon ion irradiation caused a dramatic increase in p21 expression and drastic decrease in Bax expression compared with X-irradiation. The data implicated that phospholylation of BRCA1 on serine residue 1524 might, at least partially, induce p21 expression but repress Bax expression. Together, our results suggested that the phosphorylation of BRCA1 at Ser-1524 might contribute to the G2 phase arrest and might be an upstream signal involved in preventing apoptosis signal via upregulation of p21 and downregulation of the Bax/Bcl-2 ratio. (C) 2009 Elsevier B.V. All lights reserved.
会议录NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS
会议录出版者ELSEVIER SCIENCE BV
会议录出版地PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
语种英语
WOS研究方向Instruments & Instrumentation ; Nuclear Science & Technology ; Physics
WOS记录号WOS:000265815200034
源URL[http://119.78.100.186/handle/113462/58472]  
专题中国科学院近代物理研究所
通讯作者Zhang, Hong
作者单位1.Gansu Tumor Hosp, Dept Radiotherapy, Lanzhou 730050, Peoples R China
2.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
3.Key Lab Heavy Ion Radiat Med Gansu Prov, Lanzhou 730000, Peoples R China
4.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式
GB/T 7714
Wang, Yan-ling,Zhang, Hong,Li, Ning,et al. Potential mechanisms involved in resistant phenotype of MCF-7 breast carcinoma cells to ionizing radiation induced apoptosis[C]. 见:.

入库方式: OAI收割

来源:近代物理研究所

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