Artificial creation of Chlorella pyrenoidosa mutants for economic sustainable food production
文献类型:期刊论文
作者 | Song, Xiaojin1; Wang, Jie3; Wang, Yanchao1; Feng, Yingang1; Cui, Qiu1; Lu, Yandu2 |
刊名 | BIORESOURCE TECHNOLOGY
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出版日期 | 2018-11 |
卷号 | 268页码:340-345 |
关键词 | Chlorella Functional food Heavy-ion irradiation mutagenesis Mutant creation Sweet sorghum juice |
ISSN号 | 0960-8524 |
DOI | 10.1016/j.biortech.2018.08.007 |
英文摘要 | To improve the economic viability of Chlorella as feedstock for food commodities, a serial of concentrations of low-cost sweet sorghum juice (SSJ), alternative to glucose, were used for the fermentation of Chlorella pyrenoidosa. A high biomass and protein production (8.91 g L (-1) biomass and 4.52 g L (-1) protein) was revealed with 20% SSJ. To further increase productivity, heavy-ion irradiation-mediated mutagenesis was employed to create mutants where a strain K05, with desired phenotypes (increased biomass and protein production in pilot-scale fermentation), was screened. Compared with the parental strain, the production of biomass, proteins, and chlorophylls of mutant K05 increased by 11.6%, 31.8%, and 7.6%, respectively. Production capacities under industrial scale (two-ton) further pinpoint the stability and scalability of mutant K05. These results suggest that advances in cultivation techniques coupled with artificial strain improvement will further promote microalgae as an attractive platform of functional food. |
WOS关键词 | SWEET SORGHUM JUICE ; LIPID PRODUCTIVITY ; HETEROTROPHIC CULTIVATION ; MIXOTROPHIC CULTIVATION ; HEAVY-IONS ; NANNOCHLOROPSIS ; ACID ; OPTIMIZATION ; MUTAGENESIS ; IMPROVEMENT |
资助项目 | General Financial Grant from the China Postdoctoral Science Foundation[2016M602211] |
WOS研究方向 | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
语种 | 英语 |
WOS记录号 | WOS:000445043600043 |
出版者 | ELSEVIER SCI LTD |
源URL | [http://119.78.100.186/handle/113462/59662] ![]() |
专题 | 中国科学院近代物理研究所 |
作者单位 | 1.Chinese Acad Sci, Shandong Prov Key Lab Energy Genet, CAS Key Lab Biofuels, Qingdao Engn Lab Single Cell Oil,Qingdao Inst Bio, Qingdao 266101, Shandong, Peoples R China 2.Hainan Univ, State Key Lab Marine Resource Utilizat South Chin, Coll Oceanol, Haikou 570228, Hainan, Peoples R China 3.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Gansu, Peoples R China |
推荐引用方式 GB/T 7714 | Song, Xiaojin,Wang, Jie,Wang, Yanchao,et al. Artificial creation of Chlorella pyrenoidosa mutants for economic sustainable food production[J]. BIORESOURCE TECHNOLOGY,2018,268:340-345. |
APA | Song, Xiaojin,Wang, Jie,Wang, Yanchao,Feng, Yingang,Cui, Qiu,&Lu, Yandu.(2018).Artificial creation of Chlorella pyrenoidosa mutants for economic sustainable food production.BIORESOURCE TECHNOLOGY,268,340-345. |
MLA | Song, Xiaojin,et al."Artificial creation of Chlorella pyrenoidosa mutants for economic sustainable food production".BIORESOURCE TECHNOLOGY 268(2018):340-345. |
入库方式: OAI收割
来源:近代物理研究所
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