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A porous polyaniline nanotube sorbent for solid-phase extraction of the fluorescent reaction product of reactive oxygen species in cells, and its determination by HPLC

文献类型：期刊论文

作者Niu, Panhong1,2,3; Li, Feifei3,4; Liang, Xiaojing1,2; Hou, Xiudan1,2,3; Lu, Xiaofeng1,2; Wang, Xusheng1,2; Li, Qiang4; Guo, Yong1,2
刊名MICROCHIMICA ACTA
出版日期2018-10
卷号185页码:468
关键词SPE ROS 2 ',7 '-dichlorofluorescein Doxorubicin X-ray radiation HepG2 cells Hela cells
ISSN号0026-3672
DOI10.1007/s00604-018-3000-6
英文摘要A method is described for extracting and detecting the fluorescent reaction product (2,7-dichlorofluorescein, DCF) that is formed by reaction of reactive oxygen species (ROS) with dichlorodihydrofluorescein diacetate (DCFH-DA). DCF is extracted by using porous polyaniline nanotubes (PPN) which have a large specific surface and pore volume which favor the adsorption capacity. Additional attractive features include an appropriate pore size distribution, hydrophobic surface, and electron-attracting groups which contribute to DCF adsorption. A variety of methods was applied to characterize the morphology of PPN. Under optimal conditions and by performing DCF in 0.08-1.0M concentrations, the correlation coefficient of the calibration plot is 0.999. The limits of detection for standard DCF solutions is 20nM. Compared with commercial sorbents for solid-phase extraction (SPE) such as commercially available carbon or Welchrom (R) C18, the use of the new sorbent results in better retraction recovery (92%) and longer reuse times (30 times). Doxorubicin and X-ray radiation were used to externally stimulate the ROS production in HepG2 and Hela cells. ROS was stabled by DCFH-DA and quantified by DCF. Following SPE, DCF was detected by HPLC and the concentration ROS was calculated.
WOS关键词OXIDATIVE STRESS ; SUPEROXIDE ANION ; LIVING CELLS ; HEPG2 CELLS ; APOPTOSIS ; ELECTROPHORESIS ; CARDIOTOXICITY ; ADSORBENTS ; ACTIVATION ; GENERATION
资助项目National Natural Science Foundation of China[21575149] ; National Natural Science Foundation of China[21505146] ; National Natural Science Foundation of China[21575148]
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000445106500001
出版者SPRINGER WIEN
源URL[http://119.78.100.186/handle/113462/59697]  
专题中国科学院近代物理研究所
通讯作者Liang, Xiaojing; Guo, Yong
作者单位1.Chinese Acad Sci, Lanzhou Inst Chem Phys, CAS Key Lab Chem Northwestern Plant Resource, Lanzhou 730000, Gansu, Peoples R China
2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Gansu, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Dept Med Phys, Inst Modern Phys, Lanzhou 730000, Gansu, Peoples R China
推荐引用方式
GB/T 7714		 Niu, Panhong,Li, Feifei,Liang, Xiaojing,et al. A porous polyaniline nanotube sorbent for solid-phase extraction of the fluorescent reaction product of reactive oxygen species in cells, and its determination by HPLC[J]. MICROCHIMICA ACTA,2018,185:468.
APA Niu, Panhong.,Li, Feifei.,Liang, Xiaojing.,Hou, Xiudan.,Lu, Xiaofeng.,...&Guo, Yong.(2018).A porous polyaniline nanotube sorbent for solid-phase extraction of the fluorescent reaction product of reactive oxygen species in cells, and its determination by HPLC.MICROCHIMICA ACTA,185,468.
MLA Niu, Panhong,et al."A porous polyaniline nanotube sorbent for solid-phase extraction of the fluorescent reaction product of reactive oxygen species in cells, and its determination by HPLC".MICROCHIMICA ACTA 185(2018):468.

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来源：近代物理研究所

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