Changes in cell cycle and expression of P53, MDM2 and P21 in SMMC-7721 cells irradiated by 12C6+ ions
文献类型：期刊论文
| 作者 | Wang, Yanling ; Zhang, Hong; Duan, Xin; Liu, Bing; Zhou, Qingming; Li, Xiaoda; Hao, Jifang; Liu, Hui; Zhao, Weiping
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| 刊名 | Fushe Yanjiu yu Fushe Gongyi Xuebao/Journal of Radiation Research and Radiation Processing
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| 出版日期 | 2006 |
| 卷号 | 24页码:313-316 |
| ISSN号 | 1000-3436 |
| 英文摘要 | To investigate the effect of 12C6+ ions radiation on the cell cycle and the expression of P53, MDM2 and P21 of SMMC-7721 cells. SMMC-7721 cells were irradiated with 12C6+ ions with the doses of O Gy, 1.0 Gy, 2.0 Gy, 4.0 Gy or 6.0 Gy, then dose-survival curve of SMMC-7721 cells was figured by colony-forming assay. At 24 h after irradiation cell cycle was analyzed by FACS and the expressions of P53, MDM2 and P21 were detected by Western-blot Assay. Results showed that the percentage of survival SMMC-7721 cells decreased with irradiation doses. The percentage of cells in G0/G1 phase increased after 1.0 Gy, 4.0 Gy, 6.0 Gy 12C6+ ions irradiation compared with that of the control group. However, 2.0 Gy 12C6+ ions irradiation increased the percentage of cells in G2/M phase. Compared to control, there were no changes in the expression of 57kD MDM2 in irradiated groups, but the expression levels of 76 kD MDM2, P53 and P21 increased with irradiation doses. Therefore, the different doses of 12C6+ ions irradiation can activate different cell cycle checkpoints. The increased percentages of cells in G0/G1 phase were associated with the high expression levels of P53, P21 and 76 kD MDM2 proteins. |
| 出版者 | Shanghai Science and Technology Press, Shanghai, China |
| 源URL | [http://119.78.100.186/handle/113462/64168]  |
| 专题 | 中国科学院近代物理研究所 |
| 作者单位 | 1.Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China 2.Graduate School, Chinese Academy of Sciences, Beijing 100049, China |
| 推荐引用方式 GB/T 7714 | Wang, Yanling,Zhang, Hong,Duan, Xin,et al. Changes in cell cycle and expression of P53, MDM2 and P21 in SMMC-7721 cells irradiated by 12C6+ ions[J]. Fushe Yanjiu yu Fushe Gongyi Xuebao/Journal of Radiation Research and Radiation Processing,2006,24:313-316. |
| APA | Wang, Yanling.,Zhang, Hong.,Duan, Xin.,Liu, Bing.,Zhou, Qingming.,...&Zhao, Weiping.(2006).Changes in cell cycle and expression of P53, MDM2 and P21 in SMMC-7721 cells irradiated by 12C6+ ions.Fushe Yanjiu yu Fushe Gongyi Xuebao/Journal of Radiation Research and Radiation Processing,24,313-316. |
| MLA | Wang, Yanling,et al."Changes in cell cycle and expression of P53, MDM2 and P21 in SMMC-7721 cells irradiated by 12C6+ ions".Fushe Yanjiu yu Fushe Gongyi Xuebao/Journal of Radiation Research and Radiation Processing 24(2006):313-316. |
入库方式： OAI收割
来源：近代物理研究所
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