Preliminary study on bystander effects and the mechanisms mediated through irradiated conditioned medium
文献类型：期刊论文
| 作者 | Tu, Xumin; Lei, Suwen; Li, Wenjian ; Wang, Jufang; Lu, Huimin; Zhang, Zhixing; Zhang, Cuilan; Qi, Xuesong; Hao, Shuxia; Liu, Hongwu
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| 刊名 | Fushe Yanjiu yu Fushe Gongyi Xuebao/Journal of Radiation Research and Radiation Processing
 |
| 出版日期 | 2005 |
| 卷号 | 23页码:254-256 |
| ISSN号 | 1000-3436 |
| 英文摘要 | The work is to study the bystander effects and associated mechanisms by irradiated conditioned medium (ICM) method. Splenic natural killer (NK) cells were obtained from healthy male ICR strain mice. ICM irradiated to different doses with 60Co γ-rays was used for culturing Yac-I Lymphoma cells. The degree of injury of latter by activated NK cells was observed. And effect of ICM on splenic lymphocyte proliferation was detected. Some samples of the culture media were pretreated 1 h before the irradiation with 1% DMSO, which is a scavenger of reactive oxygen species (ROS), so as to investigate possible mechanisms of a radiation-induced bystander response. The results showed that severer injury was induced in Yac-I cells cultured in ICM than the control (without irradiation), as shown by increased sensitivity to murine splenic NK cells (p less than or equal 0.05). And the splenic lymphocyte proliferation de-creased significantly than the control (p less than or equal 0.05). Culturing Yac-I cells in DMSO-pretreated medium considerably reduced the activation of NK cells. The result indicates the existence of bystander effects. It can be expected that DMSO can partly suppress ROS-induced bystander effect. This suggested ICM of Yac-I cells can trigger bystander effect. ROS may play an important role in radiation-induced bystander effect that can be partly suppressed by pre-treatment with DMSO. ROS-induced bystander effect is seen as an adjunct mechanism but not as an essential one. |
| 出版者 | Shanghai Science and Technology Press, Shanghai, China |
| 源URL | [http://119.78.100.186/handle/113462/64255]  |
| 专题 | 中国科学院近代物理研究所 |
| 作者单位 | 1.National Institute for Radiological Protection and Nuclear Safety, China CDC, Beijing 100088, China 2.Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China |
| 推荐引用方式 GB/T 7714 | Tu, Xumin,Lei, Suwen,Li, Wenjian,et al. Preliminary study on bystander effects and the mechanisms mediated through irradiated conditioned medium[J]. Fushe Yanjiu yu Fushe Gongyi Xuebao/Journal of Radiation Research and Radiation Processing,2005,23:254-256. |
| APA | Tu, Xumin.,Lei, Suwen.,Li, Wenjian.,Wang, Jufang.,Lu, Huimin.,...&Liu, Hongwu.(2005).Preliminary study on bystander effects and the mechanisms mediated through irradiated conditioned medium.Fushe Yanjiu yu Fushe Gongyi Xuebao/Journal of Radiation Research and Radiation Processing,23,254-256. |
| MLA | Tu, Xumin,et al."Preliminary study on bystander effects and the mechanisms mediated through irradiated conditioned medium".Fushe Yanjiu yu Fushe Gongyi Xuebao/Journal of Radiation Research and Radiation Processing 23(2005):254-256. |
入库方式： OAI收割
来源：近代物理研究所
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