Lipidomics Studies on Mitochondrial Damage of Saccharomyces cerevisiae Induced by Heavy Ion Beam Radiation
文献类型：期刊论文
| 作者 | Zhang Miao-Miao1,2,3 ; Guo Xiao-Peng1,2; Liu Rui-Yuan1; Ma Liang1,3; Gao Yue1,2; Lu Dong1,3; Li Wen-Jian1,3
|
| 刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2018-11 |
| 卷号 | 46页码:1714-1723 |
| ISSN号 | 0253-3820 |
| 关键词 | Lipidomics Heavy ion beam Mitochondria damage Saccharomyces cerevisiae Utra high performance liquid chromatography-electrospray ionization-mass spectrometry |
| DOI | 10.1016/S1872-2040(18)61123-5 |
| 英文摘要 | Ultra high performance liquid chromatography-electrospray ionization-mass spectrometry (UHPLC-ESI-MS) technique combined with multivariate and univariate data analysis methods was used to analyze lipid metabolomics of mitochondria-damaged Saccharomyces cerevisiae (ResD-4) after heavy ion beam radiation. The composition of lipid metabolites in Control and ResD-4 was detected, and a total of 648 kinds of lipid molecules were identified. The results showed that the orthogonal partial least squares discriminant analysis (OPLS-DA) could accurately distinguish the lipid metabolites of ResD-4 and Control. On the basis of variable importance for the projection (VIP), variation multiple (FC) and difference analysis (p), 12 subfamilies, 54 types of significant difference in lipid molecules were finally screened including DG (16:0/18:1), LPC (18:1), LPE (16:0), LPI (18:1), LPS (18:1), PA (16:0/18:1), PC (31:1), PE (16:0/10:0), PI (15:0/18:1), PS (16:0/16:1), SQDG (37:4), TG (15:0/16:0/18:1), etc. Significant differences in the expression level of lipid molecules showed that the expression levels of DG, TG, SQDG, PA, PC, PI, LPC, LPE and LPI were up-regulated, and PE, PS and LPS were down-regulated. The results showed that S. cerevisiae might respond by adjusting the content of lipid components after mitochondrial damage caused by heavy ion beam radiation. The lipid molecules with significant difference expression level might be potential markers for S. cerevisiae mitochondrial damage induced by heavy ion beam radiation. |
| WOS关键词 | DIHYDROXYACETONE PHOSPHATE ; MEMBRANE ; BIOSYNTHESIS ; MATTER ; LIPIDS |
| 资助项目 | Key Deployment Project of Chinese Academy of Sciences[KFZD-SW-109] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE INC |
| WOS记录号 | WOS:000451327200003 |
| 源URL | [http://119.78.100.186/handle/113462/64416]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Lu Dong; Li Wen-Jian |
| 作者单位 | 1.Chinese Acad Sci, Inst Modern Phys, Lanzhou 73000, Gansu, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Gansu Prov Key Lab Microbial Resources Exploit &, Lanzhou 73000, Gansu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang Miao-Miao,Guo Xiao-Peng,Liu Rui-Yuan,et al. Lipidomics Studies on Mitochondrial Damage of Saccharomyces cerevisiae Induced by Heavy Ion Beam Radiation[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2018,46:1714-1723. |
| APA | Zhang Miao-Miao.,Guo Xiao-Peng.,Liu Rui-Yuan.,Ma Liang.,Gao Yue.,...&Li Wen-Jian.(2018).Lipidomics Studies on Mitochondrial Damage of Saccharomyces cerevisiae Induced by Heavy Ion Beam Radiation.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,46,1714-1723. |
| MLA | Zhang Miao-Miao,et al."Lipidomics Studies on Mitochondrial Damage of Saccharomyces cerevisiae Induced by Heavy Ion Beam Radiation".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 46(2018):1714-1723. |
入库方式： OAI收割
来源：近代物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。