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Early embryonic exposure of ionizing radiations disrupts zebrafish pigmentation

文献类型：期刊论文

作者Wang, Yupei1,2,3,4; Zhou, Xin5; Zhao, Baoquan6; Ren, Xiaotang7; Chen, Yuhong1,2,3,4; Si, Jing1,2,3; Zhou, Rong1,2,3; Gan, Lu1,2,3; Zhang, Hong1,2,3,8
刊名JOURNAL OF CELLULAR PHYSIOLOGY
出版日期2019-01
卷号234页码:940-949
关键词ATP synthase ionizing radiation pigmentation zebrafish
ISSN号0021-9541
DOI10.1002/jcp.26922
英文摘要Studies have demonstrated that zebrafish are powerful tools for monitoring environmental toxicity, including radiation hazard. Here we investigated the developmental toxicity of ionizing radiation (IR) in an in vivo embryonic zebrafish model. The effects of heavy ion (C-12(6+)), proton, and X-ray radiation on early zebrafish embryos were determined. A similar dose-dependent decrease in the hatch and survival rate of zebrafish embryos was observed after exposure to these irradiations. Exposure of zebrafish embryos to 1-4 Gy IR caused significant loss of pigmentation. Quantitative real-time reverse transcription polymerase chain reaction, western blot analysis, and in situ hybridization (ISH) experiment revealed that atp5 alpha 1 was markedly upregulated in irradiated zebrafish embryos. In addition, IR resulted in a rapid decrease in total adenosine triphosphate (ATP) generation. With dual functions of synthesizing or hydrolyzing ATP, ATP synthase regulated H+ transport crossing the mitochondrial inner. Administration of the mitochondrial ATP synthase inhibitor, oligomycin, partially restored pigmentation in irradiated zebrafish embryos, but the ATPase inhibitor, BTB06584, had no effect. Taken together, these results showed that IR exposure downregulated zebrafish pigmentation through regulation of H+ ion transport in mitochondria.
WOS关键词GAMMA-IRRADIATION ; EXPRESSION ; IDENTIFICATION ; ACCIDENT ; ATPASE ; GENES ; CELLS ; MODEL
资助项目National Natural Science Foundation of China[11575262] ; National Natural Science Foundation of China[11605255] ; National Natural Science Foundation of China[11505244]
WOS研究方向Cell Biology ; Physiology
语种英语
WOS记录号WOS:000451533300079
出版者WILEY
源URL[http://119.78.100.186/handle/113462/64436]  
专题中国科学院近代物理研究所
通讯作者Zhang, Hong
作者单位1.Chinese Acad Sci, Inst Modern Phys, 509 Nanchang Rd, Lanzhou 730000, Gansu, Peoples R China
2.Chinese Acad Sci, Inst Modern Phys, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou, Gansu, Peoples R China
3.Inst Modern Phys, Key Lab Heavy Ion Radiat Med Gansu Prov, Lanzhou, Gansu, Peoples R China
4.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China
5.Umea Univ, Dept Med Biochem & Biophys, Umea, Sweden
6.Acad Mil Med Sci, Inst Pharmacol & Toxicol, State Key Lab Toxicol & Med Countermeasures, Beijing, Peoples R China
7.Peking Univ, State Key Lab Nucl Phys & Technol, Beijing, Peoples R China
8.Gansu Wuwei Tumor Hosp, Wuwei, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Yupei,Zhou, Xin,Zhao, Baoquan,et al. Early embryonic exposure of ionizing radiations disrupts zebrafish pigmentation[J]. JOURNAL OF CELLULAR PHYSIOLOGY,2019,234:940-949.
APA Wang, Yupei.,Zhou, Xin.,Zhao, Baoquan.,Ren, Xiaotang.,Chen, Yuhong.,...&Zhang, Hong.(2019).Early embryonic exposure of ionizing radiations disrupts zebrafish pigmentation.JOURNAL OF CELLULAR PHYSIOLOGY,234,940-949.
MLA Wang, Yupei,et al."Early embryonic exposure of ionizing radiations disrupts zebrafish pigmentation".JOURNAL OF CELLULAR PHYSIOLOGY 234(2019):940-949.

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来源：近代物理研究所

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