Determining survival fractions of Saccharomyces cerevisiae in response to ionizing radiation in liquid culture
文献类型：期刊论文
| 作者 | Guo, Xiaopeng1,2; Zhang, Miaomiao1,2 ; Gao, Yue1,2; Li, Wenjian1; Lu, Dong1
|
| 刊名 | JOURNAL OF RADIATION RESEARCH
 |
| 出版日期 | 2018-11 |
| 卷号 | 59页码:760-764 |
| ISSN号 | 0449-3060 |
| 关键词 | survival fractions Saccharomyces cerevisiae X-ray radiation OD600 96-well plate |
| DOI | 10.1093/jrr/rry070 |
| 英文摘要 | Saccharomyces cerevisiae survival fractions (SFs) in response to X-ray radiation were determined by two new methods: an OD600-based method and a 96-well method. For the OD600-based method, cells were exposed to various X-ray doses and inoculated into fresh medium: a lower biomass accumulated, indicating fewer surviving cells within the investigated dose range (0-100 Gy). For the 96-well method, diluent containing similar to 0-100 cells was equally divided into 96 droplets and respectively inoculated into 96 wells containing 200 mu l of broth: fewer wells without S. cerevisiae clones indicated more surviving cells after 48 h of incubation. Corresponding quantitative systems were established. Both methods were sensitive and reliable. The OD600-based method is simple and fast, and the 96-well method simplifies the counting process. SF estimates by the OD600-based method were lower than those by 96-well methods owing to cell cycle arrest. In addition, comparisons of newly proposed and plate-counting methods indicated a higher rate of repair in S. cerevisiae in liquid culture than on agar. |
| 资助项目 | National Natural Science Fund of China[11575259] |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics ; Oncology ; Radiology, Nuclear Medicine & Medical Imaging |
| 语种 | 英语 |
| 出版者 | OXFORD UNIV PRESS |
| WOS记录号 | WOS:000452745600007 |
| 源URL | [http://119.78.100.186/handle/113462/64460]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Lu, Dong |
| 作者单位 | 1.Chinese Acad Sci, Inst Modern Phys, 509 Nanchang Rd, Lanzhou 730000, Gansu, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Xiaopeng,Zhang, Miaomiao,Gao, Yue,et al. Determining survival fractions of Saccharomyces cerevisiae in response to ionizing radiation in liquid culture[J]. JOURNAL OF RADIATION RESEARCH,2018,59:760-764. |
| APA | Guo, Xiaopeng,Zhang, Miaomiao,Gao, Yue,Li, Wenjian,&Lu, Dong.(2018).Determining survival fractions of Saccharomyces cerevisiae in response to ionizing radiation in liquid culture.JOURNAL OF RADIATION RESEARCH,59,760-764. |
| MLA | Guo, Xiaopeng,et al."Determining survival fractions of Saccharomyces cerevisiae in response to ionizing radiation in liquid culture".JOURNAL OF RADIATION RESEARCH 59(2018):760-764. |
入库方式： OAI收割
来源：近代物理研究所
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