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Heavy-ion mutagenesis significantly enhances enduracidin production by Streptomyces fungicidicus

文献类型：期刊论文

作者Liu, Lu1,2; Hu, Wei1; Li, Wen-jian1; Wang, Shu-yang1; Lu, Dong1; Tian, Xue-jiao1,2; Mao, Yan-qin1,2; Liu, Jing1; Chen, Ji-hong1
刊名ENGINEERING IN LIFE SCIENCES
出版日期2019-02-01
卷号19页码:112-120
关键词fermentation industry heavy ion mutagenesis mutation tool screening process Streptomyces fungicidicus
ISSN号1618-0240
DOI10.1002/elsc.201800109
英文摘要To improve fermentative production of enduracidin, heavy-ion beams generated by the Heavy Ion Research Facility in Lanzhou (HIRFL), China, were employed for the first time to generate mutations in Streptomyces fungicidicus. Initial screening detected 44 positive mutants with larger inhibition zone, which were subsequently tested based on flask fermentation. Finally, 20 mutants showed 20% increase in enduracidin production, when compared with the original strain. Among them, enduracidin production by the three mutants (M13, M30, and M34) was significantly higher than that by the original strain. In particular, mutant M30 exhibited highest enduracidin production, which was 114% higher than that obtained with the original strain. Following culture optimization, the maximal enduracidin yield obtained by M30 reached 918.5 mg/L in 10 days, which was 34% higher than that noted in the control.
WOS关键词MUTATION ; BEAMS
资助项目National Natural Science Foundation of China[11605259] ; western talents program of the Chinese Academy of Sciences[Y706030XB0] ; Open Funds of the Key Laboratory of Development and Application of Rural Renewable Energy, Ministry of Agriculture, China[2017002]
WOS研究方向Biotechnology & Applied Microbiology
语种英语
WOS记录号WOS:000457650400003
出版者WILEY
资助机构National Natural Science Foundation of China ; western talents program of the Chinese Academy of Sciences ; Open Funds of the Key Laboratory of Development and Application of Rural Renewable Energy, Ministry of Agriculture, China
源URL[http://119.78.100.186/handle/113462/65172]  
专题中国科学院近代物理研究所
作者单位1.Chinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式
GB/T 7714		 Liu, Lu,Hu, Wei,Li, Wen-jian,et al. Heavy-ion mutagenesis significantly enhances enduracidin production by Streptomyces fungicidicus[J]. ENGINEERING IN LIFE SCIENCES,2019,19:112-120.
APA Liu, Lu.,Hu, Wei.,Li, Wen-jian.,Wang, Shu-yang.,Lu, Dong.,...&Chen, Ji-hong.(2019).Heavy-ion mutagenesis significantly enhances enduracidin production by Streptomyces fungicidicus.ENGINEERING IN LIFE SCIENCES,19,112-120.
MLA Liu, Lu,et al."Heavy-ion mutagenesis significantly enhances enduracidin production by Streptomyces fungicidicus".ENGINEERING IN LIFE SCIENCES 19(2019):112-120.

入库方式： OAI收割

来源：近代物理研究所

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