Impact of carrier stiffness and microtopology on two-dimensional kinetics of P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) interactions
文献类型：期刊论文
| 作者 | Wu L; Xiao BT; Jia XL(贾潇凌) ; Zhang Y(章燕); Lv SQ(吕守芹); Chen J(陈娟); Long M(龙勉)
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| 刊名 | Journal of Biological Chemistry
 |
| 出版日期 | 2007 |
| 卷号 | 282期号:13页码:9846-9854 |
| 通讯作者邮箱 | mlong@imech.ac.cn |
| ISSN号 | 0021-9258 |
| 关键词 | Human Neutrophils Binding-Kinetics Adhesion Affinity Rates Flow |
| 通讯作者 | Long, M (reprint author), Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Beijing 100080, Peoples R China. |
| 中文摘要 | Mechanics and surface microtopology of the molecular carrier influence cell adhesion, but the mechanisms underlying these effects are not well understood. We used a micropipette adhesion frequency assay to quantify how the carrier stiffness and microtopology affected two-dimensional kinetics of interacting adhesion molecules on two apposing surfaces. Interactions of P-selectin with P-selectin glycoprotein ligand-1 (PSGL-1) were used to demonstrate such effects by presenting the molecules on three carrier systems: human red blood cells (RBCs), human promyelocytic leukemia HL-60 cells, and polystyrene beads. Stiffening the carrier alone or in cooperation with roughing the surface lowered the two-dimensional affinity of interacting molecules by reducing the forward rate but not the reverse rate, whereas softening the carrier and roughing the surface had opposing effects in affecting two-dimensional kinetics. In contrast, the soluble antibody bound with similar three-dimensional affinity to surface-anchored P-selectin or PSGL-1 constructs regardless of carrier stiffness and microtopology. These results demonstrate that the carrier stiffness and microtopology of a receptor influences its rate of encountering and binding a surface ligand but does not subsequently affect the stability of binding. This provides new insights into understanding the rolling and tethering mechanism of leukocytes onto endothelium in both physiological and pathological processes. |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | HUMAN NEUTROPHILS ; BINDING-KINETICS ; ADHESION ; AFFINITY ; RATES ; FLOW |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000245421700059 |
| 公开日期 | 2009-08-03 ; 2010-06-13 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/34011]  |
| 专题 | 力学研究所_力学所知识产出(1956-2008) |
| 推荐引用方式 GB/T 7714 | Wu L,Xiao BT,Jia XL,et al. Impact of carrier stiffness and microtopology on two-dimensional kinetics of P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) interactions[J]. Journal of Biological Chemistry,2007,282(13):9846-9854. |
| APA | Wu L.,Xiao BT.,贾潇凌.,章燕.,吕守芹.,...&龙勉.(2007).Impact of carrier stiffness and microtopology on two-dimensional kinetics of P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) interactions.Journal of Biological Chemistry,282(13),9846-9854. |
| MLA | Wu L,et al."Impact of carrier stiffness and microtopology on two-dimensional kinetics of P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) interactions".Journal of Biological Chemistry 282.13(2007):9846-9854. |
入库方式： OAI收割
来源：力学研究所
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