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Cellulase production by Aspergillus fumigatus MS13.1 mutant generated by; heavy ion mutagenesis and its efficient saccharification of pretreated sweet sorghum straw

文献类型：期刊论文

作者Li, Wenjian1,2; Dong, Miaoyin1,2; Xiao, Guoqing2,3; Wang, Shuyang1,2; Xua, Fuqiang1,2; Hua, Wei1,2; Li, Qiaoqiao1,2; Chen, Jihong1,2
刊名PROCESS BIOCHEMISTRY
出版日期2019-09-01
卷号84页码:22-29
关键词Aspergillus fumigatus Cellulase Heavy ion mutagenesis Enzymatic saccharification Sweet sorghum straw
ISSN号1359-5113
DOI10.1016/j.procbio.2019.06.006
通讯作者Wang, Shuyang(wangsy@impcas.ac.cn)
英文摘要Developing a potential strain with high cellulase yield and stable fermenting performance is an imperative way to overcome the enzyme costs limitation. A cellulose degrading fungus was isolated from the agricultural wastes and identified as A. fumigatus MS13.1. For further improving the production capacity of enzyme, the original A. fumigatus MS13.1 was subjected to mutagenesis with heavy-ion beams and mutant MS160.53 was selected. The MS160.53 exhibited higher filter paper (1.81 IU/ml) and beta-glucosidase (1.65 IU/ml) activities than parent strain MS13.1, which increased by 40.3% and 15.1%, respectively. Soluble protein concentrations in supernatants from strain MS160.53 were reached 1.497 mg/ml at 240 h which were higher than that in the reported literature of A. fumigatus. In addition, the SDS-PAGE profiles revealed that more enzymes of mutant were secreted during fermentation compared with parent strain MS131.1. The crude enzyme were further applied for saccharification of pretreated sweet sorghum straw, where a dosage of 5 IU/g FPase produced high reducing sugar titers of 789.75 mg/g. SEM images revealed the obviously microstructural changes of straw after the pretreatment and enzymatic hydrolysis. Thus, the enzyme cocktails produced by A. fumigates MS160.53 has great potential for the saccharification of pretreated biomass and further bioethanol production.
WOS关键词TRICHODERMA-REESEI ; RICE STRAW ; ENZYMATIC SACCHARIFICATION ; AGROINDUSTRIAL RESIDUES ; CELLULOLYTIC ENZYMES ; BATCH CULTURE ; HYDROLYSIS ; BIOMASS ; FUNGI ; FERMENTATION
资助项目National Natural Science Foundation of China[11305225] ; Chinese Academy of Sciences[KFZD-SW-109] ; Xizang forage Science and Technology[2016ZDKJZC-22]
WOS研究方向Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering
语种英语
WOS记录号WOS:000481724000003
出版者ELSEVIER SCI LTD
资助机构National Natural Science Foundation of China ; Chinese Academy of Sciences ; Xizang forage Science and Technology
源URL[http://119.78.100.186/handle/113462/132874]  
专题中国科学院近代物理研究所
通讯作者Wang, Shuyang
作者单位1.Univ Chinese Acad Sci, Coll Life Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Inst Modern Phys, 509 Nanchang Rd, Lanzhou 730000, Gansu, Peoples R China
3.Univ Chinese Acad Sci, Sch Nucl Sci & Technol, 19A Yuquan Rd, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Li, Wenjian,Dong, Miaoyin,Xiao, Guoqing,et al. Cellulase production by Aspergillus fumigatus MS13.1 mutant generated by; heavy ion mutagenesis and its efficient saccharification of pretreated sweet sorghum straw[J]. PROCESS BIOCHEMISTRY,2019,84:22-29.
APA Li, Wenjian.,Dong, Miaoyin.,Xiao, Guoqing.,Wang, Shuyang.,Xua, Fuqiang.,...&Chen, Jihong.(2019).Cellulase production by Aspergillus fumigatus MS13.1 mutant generated by; heavy ion mutagenesis and its efficient saccharification of pretreated sweet sorghum straw.PROCESS BIOCHEMISTRY,84,22-29.
MLA Li, Wenjian,et al."Cellulase production by Aspergillus fumigatus MS13.1 mutant generated by; heavy ion mutagenesis and its efficient saccharification of pretreated sweet sorghum straw".PROCESS BIOCHEMISTRY 84(2019):22-29.

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来源：近代物理研究所

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