Induction of cancer stem-like cells in A549 cells after exposure to carbon ions and X-rays
文献类型：期刊论文
| 作者 | Hu, B.1,3; Li, X.1,2,3 ; Jia, R.1,2,3; Du, Y.1,3; Pan, D.1,2,3; Chen, Y.1,3
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| 刊名 | INTERNATIONAL JOURNAL OF RADIATION RESEARCH
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| 出版日期 | 2019 |
| 卷号 | 17期号:1页码:25-35 |
| 关键词 | Cancer stem-like cells (CSCs) CD133 low and high LET radiation carcinogenesis |
| ISSN号 | 2322-3243 |
| DOI | 10.18869/acadpub.ijrr.17.1.25 |
| 通讯作者 | Hu, B.(hubr@impcas.ac.cn) |
| 英文摘要 | Background: Cancer stem-like cells (CSCs) play a crucial role in the initiation, progression, and recurrence of cancer. Evidence indicates that the high linear energy transfer (LET) carbon ion beam is more effective against CSCs than the conventional X-ray beam. Carbon ion radiotherapy is considered as a promising cancer strategy, however, information about whether, or not, new CSCs are induced after carcinoma cells exposed to carbon ions is limited and incomplete. Materials and Methods: The selected A549 cells, derived from a single colony with low CD133 expression, were irradiated by high LET carbon ion beam or low LET X-ray, and then the cell clonal survival fraction, CD133(+) expression subpopulations and soft agar colony formation potential were detected. Results: The cell-killing ability of carbon ions was higher than that of X-rays. The fractions of CD133(+) or Oct4(+) high expression cells increased in a dose-dependent manner at 12 h after both X-ray and carbon ion exposure. Compared to that of carbon ion irradiation, the efficiency of transformation in a cell colony was higher in the X-ray irradiation group after 30 d; however, the amount of colonies with higher fractions of CD133(+) expression cell subpopulation in the carbon ion irradiation group was more than that in X-ray irradiation treatment group after 30 and 50 d. Conclusion: Our results indicate that, although high-LET radiation kill more cells including cancer stem-like A549 cells, it may induce more stable stem cell-like colonies than low LET radiation. |
| WOS关键词 | CLUSTERED DNA-DAMAGE ; LUNG-CANCER ; ENDOTHELIAL-CELLS ; ALPHA-PARTICLE ; IDENTIFICATION ; RADIOTHERAPY ; EXPRESSION ; CD133 ; RADIORESISTANCE ; IRRADIATION |
| 资助项目 | National Natural Science Foundation of China[31170803] ; National Natural Science Foundation of China[U1432121] ; National Natural Science Foundation of China[U1632145] ; National Key Scientific Instrument and Equipment Development Project of China[2012YQ03014210] |
| WOS研究方向 | Radiology, Nuclear Medicine & Medical Imaging |
| 语种 | 英语 |
| WOS记录号 | WOS:000464529800003 |
| 出版者 | IJRR-IRANIAN JOURNAL RADIATION RES |
| 资助机构 | National Natural Science Foundation of China ; National Key Scientific Instrument and Equipment Development Project of China |
| 源URL | [http://119.78.100.186/handle/113462/133738]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Hu, B. |
| 作者单位 | 1.Chinese Acad Sci, Inst Modern Phys, CAS Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Gansu, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Modern Phys, Gansu Key Lab Space Radiobiol, Lanzhou 730000, Gansu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, B.,Li, X.,Jia, R.,et al. Induction of cancer stem-like cells in A549 cells after exposure to carbon ions and X-rays[J]. INTERNATIONAL JOURNAL OF RADIATION RESEARCH,2019,17(1):25-35. |
| APA | Hu, B.,Li, X.,Jia, R.,Du, Y.,Pan, D.,&Chen, Y..(2019).Induction of cancer stem-like cells in A549 cells after exposure to carbon ions and X-rays.INTERNATIONAL JOURNAL OF RADIATION RESEARCH,17(1),25-35. |
| MLA | Hu, B.,et al."Induction of cancer stem-like cells in A549 cells after exposure to carbon ions and X-rays".INTERNATIONAL JOURNAL OF RADIATION RESEARCH 17.1(2019):25-35. |
入库方式： OAI收割
来源：近代物理研究所
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