Ultra-small gadolinium oxide nanocrystal sensitization of non-small-cell lung cancer cells toward X-ray irradiation by promoting cytostatic autophagy
文献类型：期刊论文
| 作者 | Wu, Aiguo1; Li, Feifei2,3; Shen, Zheyu1; Li, Ping2 ; Zhang, Pengcheng2,3; Liu, Yan2; Jin, Xiaodong2; Li, Zihou1,3; Li, Qiang2; Chen, Weiqiang2
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| 刊名 | INTERNATIONAL JOURNAL OF NANOMEDICINE
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| 出版日期 | 2019 |
| 卷号 | 14页码:2415-2431 |
| 关键词 | gadolinium oxide nanocrystal radiosensitization cytostatic autophagy apoptosis oxidative stress |
| ISSN号 | 1178-2013 |
| DOI | 10.2147/IJN.S193676 |
| 通讯作者 | Chen, Weiqiang(chenwq7315@impcas.ac.cn) ; Li, Qiang(liqiang@impcas.ac.cn) |
| 英文摘要 | Background: Gadolinium-based nanoparticles (GdNPs) have been used as theranostic sensitizers in clinical radiotherapy studies; however, the biomechanisms underlying the radiosensitizing effects of GdNPs have yet to be determined. In this study, ultra-small gadolinium oxide nanocrystals (GONs) were employed to investigate their radiosensitizing effects and biological mechanisms in non-small-cell lung cancer (NSCLC) cells under X-ray irradiation. Method and materials: GONs were synthesized using polyol method. Hydroxyl radical production, oxidative stress, and clonogenic survival after X-ray irradiation were used to evaluate the radiosensitizing effects of GONs. DNA double-strand breakage, cell cycle phase, and apoptosis and autophagy incidences were investigated in vitro to determine the radiosensitizing biomechanism of GONs under X-ray irradiation. Results: GONs induced hydroxyl radical production and oxidative stress in a dose-and concentration-dependent manner in NSCLC cells after X-ray irradiation. The sensitizer enhancement ratios of GONs ranged between 19.3% and 26.3% for the NSCLC cells under investigation with a 10% survival rate compared with that of the cells treated with irradiation alone. Addition of 3-methyladenine to the cell medium decreased the incidence rate of autophagy and increased cell survival, supporting the idea that the GONs promoted cytostatic autophagy in NSCLC cells under X-ray irradiation. Conclusion: This study examined the biological mechanisms underlying the radiosensitizing effects of GONs on NSCLC cells and presented the first evidence for the radiosensitizing effects of GONs via activation of cytostatic autophagy pathway following X-ray irradiation. |
| WOS关键词 | GOLD NANOPARTICLES ; RADIATION-THERAPY ; TUMOR-CELLS ; RADIOTHERAPY ; ENHANCEMENT ; RADIOSENSITIZATION ; METASTASES ; PROTON ; DEATH |
| 资助项目 | National Key Research and Development Program[2017YFC0108500] ; National Key Technology Support Program of the Ministry of Science and Technology of China[2015BAI01B11] ; National Natural Science Foundation of China[11875299] ; CAS Key Laboratory of Heavy Ion Radiation Biology and Medicine, Institute of Modern Physics[2016-01] |
| WOS研究方向 | Science & Technology - Other Topics ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000463728200002 |
| 出版者 | DOVE MEDICAL PRESS LTD |
| 资助机构 | National Key Research and Development Program ; National Key Technology Support Program of the Ministry of Science and Technology of China ; National Natural Science Foundation of China ; CAS Key Laboratory of Heavy Ion Radiation Biology and Medicine, Institute of Modern Physics |
| 源URL | [http://119.78.100.186/handle/113462/133839]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Li, Qiang; Chen, Weiqiang |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Magnet Mat & Devices, Ningbo Inst Mat Technol & Engn, Div Funct Mat & Nano Devices, Ningbo 315201, Zhejiang, Peoples R China 2.Chinese Acad Sci, Key Lab Basic Res Heavy Ion Radiat Applicat Med, Inst Modern Phys, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Gansu, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Aiguo,Li, Feifei,Shen, Zheyu,et al. Ultra-small gadolinium oxide nanocrystal sensitization of non-small-cell lung cancer cells toward X-ray irradiation by promoting cytostatic autophagy[J]. INTERNATIONAL JOURNAL OF NANOMEDICINE,2019,14:2415-2431. |
| APA | Wu, Aiguo.,Li, Feifei.,Shen, Zheyu.,Li, Ping.,Zhang, Pengcheng.,...&Chen, Weiqiang.(2019).Ultra-small gadolinium oxide nanocrystal sensitization of non-small-cell lung cancer cells toward X-ray irradiation by promoting cytostatic autophagy.INTERNATIONAL JOURNAL OF NANOMEDICINE,14,2415-2431. |
| MLA | Wu, Aiguo,et al."Ultra-small gadolinium oxide nanocrystal sensitization of non-small-cell lung cancer cells toward X-ray irradiation by promoting cytostatic autophagy".INTERNATIONAL JOURNAL OF NANOMEDICINE 14(2019):2415-2431. |
入库方式： OAI收割
来源：近代物理研究所
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