Flavonoid genistein protects bone marrow sinusoidal blood vessels from damage by methotrexate therapy in rats
文献类型：期刊论文
| 作者 | Su, Yu-Wen3,4; Khabbazi, Samira3,4; Fan, Chia-Ming3,4; Chen, Ke-Ming8; Hassanshahi, Mohammadhossein3,4; Qian, Airong5; Xian, Cory J.3,4; Zhou, Hou-De2; Yan, De-Wen7; Howe, Peter R.1,6 |
| 刊名 | JOURNAL OF CELLULAR PHYSIOLOGY
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| 出版日期 | 2019-07-01 |
| 卷号 | 234期号:7页码:11276-11286 |
| ISSN号 | 0021-9541 |
| 关键词 | angiogenesis apoptosis bone marrow sinusoidal endothelial cells (BM SECs) genistein |
| DOI | 10.1002/jcp.27785 |
| 通讯作者 | Xian, Cory J.(cory.xian@unisa.edu.au) |
| 英文摘要 | Cancer chemotherapy can cause significant damage to the bone marrow (BM) microvascular (sinusoidal) system. Investigations must now address whether and how BM sinusoidal endothelial cells (SECs) can be protected during chemotherapy. Herein we examined the potential protective effects of genistein, a soy-derived flavonoid, against BM sinusoidal damage caused by treatment with methotrexate (MTX). The groups of young adult rats were gavaged daily with genistein (20mg/kg) or placebo. After 1 week, rats also received daily injections of MTX (0.75mg/kg) or saline for 5 days and were killed after a further 4 days. Histological analyses showed that BM sinusoids were markedly dilated (p<0.001) in the MTX-alone group but were unaffected or less dilated in the genistein+MTX group. In control rats, genistein significantly enhanced expression of vascular endothelial growth factor (VEGF; p<0.01), particularly in osteoblasts, and angiogenesis marker CD31 (p<0.001) in bone. In MTX-treated rats, genistein suppressed MTX-induced apoptosis of BM SECs (p<0.001 vs MTX alone group) and tended to increase expression of CD31 and VEGF (p<0.05). Our in vitro studies showed that genistein in certain concentrations protected cultured SECs from MTX cytotoxic effects. Genistein enhanced tube formation of cultured SECs, which is associated with its ability to induce expression of endothelial nitric oxide synthase and production of nitric oxide. These data suggest that genistein can protect BM sinusoids during MTX therapy, which is associated, at least partially, with its indirect effect of promoting VEGF expression in osteoblasts and its direct effect of enhancing nitric oxide production in SECs. |
| WOS关键词 | NITRIC-OXIDE SYNTHASE ; PHYTOESTROGEN GENISTEIN ; EXPRESSION ; ANGIOGENESIS ; CHEMOTHERAPY ; MECHANISMS ; CELLS ; DIFFERENTIATION ; HEMATOPOIESIS ; REGENERATION |
| 资助项目 | National Natural Science Foundation of China[81671928] ; National Health and Medical Research Council[1042105] ; National Health and Medical Research Council[1127396] ; National Health and Medical Research Council[1010752] ; National Health and Medical Research Council[0508046] ; National Health and Medical Research Council[0508047] ; Research Training Program international (RTPi) scholarship of University of South Australia |
| WOS研究方向 | Cell Biology ; Physiology |
| 语种 | 英语 |
| 出版者 | WILEY |
| WOS记录号 | WOS:000462645700127 |
| 资助机构 | National Natural Science Foundation of China ; National Health and Medical Research Council ; Research Training Program international (RTPi) scholarship of University of South Australia |
| 源URL | [http://119.78.100.186/handle/113462/133997]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Xian, Cory J. |
| 作者单位 | 1.Univ Newcastle, Clin Nutr Res Ctr, Callaghan, NSW, Australia 2.Cent S Univ, Xiangya Hosp 2, Natl Clin Res Ctr Metab Dis, Dept Endocrinol & Metab, Changsha, Hunan, Peoples R China 3.Univ South Australia, Sch Pharm & Med Sci, GPO Box 2471, Adelaide, SA 5001, Australia 4.Univ South Australia, UniSA Canc Res Inst, GPO Box 2471, Adelaide, SA 5001, Australia 5.Northwestern Polytech Univ, Key Lab Space Biosci & Biotechnol, Sch Life Sci, Lab Bone Metab, Xian, Shaanxi, Peoples R China 6.Univ Southern Queensland, Inst Resilient Reg, Springfield, Qld, Australia 7.Shenzhen Univ, Affiliated Hosp 1, Dept Endocrinol, Shenzhen, Guangdong, Peoples R China 8.Lanzhou Gen Hosp CPLA, Inst Orthopaed, Lanzhou, Gansu, Peoples R China 9.Chinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Su, Yu-Wen,Khabbazi, Samira,Fan, Chia-Ming,et al. Flavonoid genistein protects bone marrow sinusoidal blood vessels from damage by methotrexate therapy in rats[J]. JOURNAL OF CELLULAR PHYSIOLOGY,2019,234(7):11276-11286. |
| APA | Su, Yu-Wen.,Khabbazi, Samira.,Fan, Chia-Ming.,Chen, Ke-Ming.,Hassanshahi, Mohammadhossein.,...&Wang, Ju-Fang.(2019).Flavonoid genistein protects bone marrow sinusoidal blood vessels from damage by methotrexate therapy in rats.JOURNAL OF CELLULAR PHYSIOLOGY,234(7),11276-11286. |
| MLA | Su, Yu-Wen,et al."Flavonoid genistein protects bone marrow sinusoidal blood vessels from damage by methotrexate therapy in rats".JOURNAL OF CELLULAR PHYSIOLOGY 234.7(2019):11276-11286. |
入库方式： OAI收割
来源：近代物理研究所
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