A Validated HPLC-MS/MS Assay for 14-O-[(4,6-Diaminopyrimidine-2-yl)thioacetyl] Mutilin in Biological Samples and Its Pharmacokinetic, Distribution and Excretion via Urine and Feces in Rats
文献类型：期刊论文
| 作者 | Shang, Ruofeng1; Fu, Yunxing1; Liu, Yu1; Yi, Yunpeng1; Liang, Jianping1 ; Wu, Qingfeng2
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| 刊名 | MOLECULES
 |
| 出版日期 | 2019-02-02 |
| 卷号 | 24期号:4页码:17 |
| 关键词 | DPTM HPLC-MS MS pharmacokinetics distribution excretion |
| ISSN号 | 1420-3049 |
| DOI | 10.3390/molecules24040790 |
| 通讯作者 | Wu, Qingfeng(wuqf@impcas.ac.cn) ; Shang, Ruofeng(shangrf1974@163.com) |
| 英文摘要 | 14-O-[(4,6-Diaminopyrimidine-2-yl)thioacetyl] mutilin (DPTM), a novel pleuromutilin candidate with a substituted pyrimidine moiety, has been confirmed to possess excellent antibacterial activity against Gram-positive bacteria. To illustrate the pharmacokinetic profile after intravenous (i.v.), intramuscular (i.m.) and oral (p.o.) administrations with DPTM, as well as tissue distribution and excretion via urine and feces in vivo, a specific, sensitive and robust HPLC-MS/MS method was first developed to determine DPTM in rat plasma, various tissues, urine and feces. The plasma, tissues, urine and feces samples were treated by protein precipitation with acetonitrile using tiamulin fumarate as an internal standard (IS). This method which was achieved on an HPLC system detector equipped with an ESI interface, was sensitive with 5 ng/mL as the lower limit of detection and exhibited good linearity (R-2 > 0.9900) in the range of 5-4000 ng/mL for plasma, various tissues, urine and feces, as well as intra-day precision, inter-day precision and accuracy. The matrix effects ranged from 94.2 to 109.7% with RSD 9.4% and the mean extraction recoveries ranged from 95.4 to 109.5% in plasma, tissue homogenates, urine and feces (RSD 9.9). After i.v., i.m. and p.o. administrations, DPTM was rapidly absorbed and metabolized in rats with the half-life (t(1/2)) of 1.70-1.86, 3.23-3.49 and 4.38-4.70 for 10, 25 and 75 mg/kg doses, respectively. The tissue distribution showed that DPTM was diffused into all the tested tissues, especially into the intestine and lung. Excretion via urine and feces studies demonstrated that DPTM was mainly excreted by feces after administration. |
| WOS关键词 | PLEUROMUTILIN DERIVATIVES ; ANTIBACTERIAL ACTIVITY ; TISSUES APPLICATION ; PLASMA ; VALNEMULIN ; TIAMULIN ; BINDING ; PROFILE |
| 资助项目 | National Natural Science Foundation of China[31873027] ; Agricultural Science and Technology Innovation Program[CAASASTIP-2014-LIHPS-04] ; National Key Technology Support Program[2015BAD11B02] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000460805900136 |
| 出版者 | MDPI |
| 资助机构 | National Natural Science Foundation of China ; Agricultural Science and Technology Innovation Program ; National Key Technology Support Program |
| 源URL | [http://119.78.100.186/handle/113462/134371]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Shang, Ruofeng; Wu, Qingfeng |
| 作者单位 | 1.CAAS, Key Lab New Anim Drug Project Gansu Prov, Key Lab Vet Pharmaceut Dev, Minist Agr,Lanzhou Inst Husb & Pharmaceut Sci, Lanzhou 730050, Gansu, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shang, Ruofeng,Fu, Yunxing,Liu, Yu,et al. A Validated HPLC-MS/MS Assay for 14-O-[(4,6-Diaminopyrimidine-2-yl)thioacetyl] Mutilin in Biological Samples and Its Pharmacokinetic, Distribution and Excretion via Urine and Feces in Rats[J]. MOLECULES,2019,24(4):17. |
| APA | Shang, Ruofeng,Fu, Yunxing,Liu, Yu,Yi, Yunpeng,Liang, Jianping,&Wu, Qingfeng.(2019).A Validated HPLC-MS/MS Assay for 14-O-[(4,6-Diaminopyrimidine-2-yl)thioacetyl] Mutilin in Biological Samples and Its Pharmacokinetic, Distribution and Excretion via Urine and Feces in Rats.MOLECULES,24(4),17. |
| MLA | Shang, Ruofeng,et al."A Validated HPLC-MS/MS Assay for 14-O-[(4,6-Diaminopyrimidine-2-yl)thioacetyl] Mutilin in Biological Samples and Its Pharmacokinetic, Distribution and Excretion via Urine and Feces in Rats".MOLECULES 24.4(2019):17. |
入库方式： OAI收割
来源：近代物理研究所
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