Effect of exogenous wild-type p53 on melanoma cell death pathways induced by irradiation at different linear energy transfer
文献类型：期刊论文
| 作者 | Min, FL; Zhang, H; Li, WJ; Gao, QX; Zhou, GM |
| 刊名 | IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
 |
| 出版日期 | 2005-09-01 |
| 卷号 | 41期号:8-9页码:284-288 |
| ISSN号 | 1071-2690 |
| 关键词 | p53 gene carbon-ion beams LET cell death melanoma |
| 英文摘要 | We investigated the effect of exogenous wild-Lype p53 on the radiation-induced cells apoptosis and necrosis at different levels of linear energy transfer (LET) to evaluate its mechanisms. The human melanoma cell line A375, which bears wild-type p53 gene status, was used, as well as the transfectant A375 cells (A375/p53) with adenoviral vector containing the wild-type p53 gene. We exposed these cells to X-rays and to accelerated carbon-ion (C-) beams. Cellular sensitivities were determined by using clonogenic assay. Apoptotic and necrotic cell deaths were determined morphologically by dual staining (acridine orange and ethidium bromide) using fluorescence microscopy. We discovered that (1) there was no significant difference in survival fraction between A375 cells and A375/p53 cells irradiated by C-beams with greater than 32 KeV/mu m LET, (2) although apoptosis in the two kinds of cells increased in an LET-dependent manner, exogenous wild-type P53 induced cell apoptosis efficiently in A375/p53 relative to A375 cells with X-rays or high-LET irradiation, and (3) by high-LEI' irradiation, the number of necrosis in A375 cells increased significantly (P < 0.05) in comparison with A375/p53 cells. These results indicate that in high-LET irradiation apoptosis induction is p53 dependent partly and exogenous wild-type P53 plays an important role in modulating cell death type, although there was no significant difference in cellular radiosensitivities. Our observation in the study offers the potential application of high-LET radiation combined with p53 in the management of human patients with melanoma. |
| WOS关键词 | CARBON ION RADIOTHERAPY ; RADIATION-INDUCED APOPTOSIS ; INHIBITION ; CARCINOMAS ; EFFICACY ; NECROSIS ; THERAPY ; CANCER ; BEAMS |
| WOS研究方向 | Cell Biology ; Developmental Biology |
| 语种 | 英语 |
| 出版者 | SOC IN VITRO BIOLOGY |
| WOS记录号 | WOS:000234638400007 |
| 公开日期 | 2010-10-29 |
| 源URL | [http://ir.imp.cas.cn/handle/113462/1049]  |
| 专题 | 近代物理研究所_近代物理研究所知识存储（2010之前） |
| 通讯作者 | Gao, QX |
| 作者单位 | 1.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Gansu Province, Peoples R China 3.Chinese Acad Sci, Sch Life Sci, Lanzhou 730000, Gansu Province, Peoples R China |
| 推荐引用方式 GB/T 7714 | Min, FL,Zhang, H,Li, WJ,et al. Effect of exogenous wild-type p53 on melanoma cell death pathways induced by irradiation at different linear energy transfer[J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL,2005,41(8-9):284-288. |
| APA | Min, FL,Zhang, H,Li, WJ,Gao, QX,&Zhou, GM.(2005).Effect of exogenous wild-type p53 on melanoma cell death pathways induced by irradiation at different linear energy transfer.IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL,41(8-9),284-288. |
| MLA | Min, FL,et al."Effect of exogenous wild-type p53 on melanoma cell death pathways induced by irradiation at different linear energy transfer".IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL 41.8-9(2005):284-288. |
入库方式： OAI收割
来源：近代物理研究所
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