Flower color chimera and abnormal leaf mutants induced by C-12(6+) heavy ions in Salvia splendens Ker-Gawl.
文献类型:期刊论文
作者 | Wu, D. L.1; Hou, S. W.1,2; Qian, P. P.1; Sun, L. D.1; Zhang, Y. C.1; Li, W. J.2 |
刊名 | SCIENTIA HORTICULTURAE
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出版日期 | 2009-08-04 |
卷号 | 121期号:4页码:462-467 |
关键词 | Salvia splendens Ker-Gawl. Leaf mutants Flower color chimera RAPD Micropropagation |
ISSN号 | 0304-4238 |
英文摘要 | The effect of C-12(6+) heavy ions bombardment on mutagenesis in Salvia splendens Ker-Gawl. was studied. Dose-response studies indicated that there was a peak of malformation frequency of S. splendens at 200 Gy. Abnormal leaf mutants of the bileaf, trileaf and tetraleaf conglutination were selected. Meanwhile, a bicolor flower chimera with dark red and fresh red flower was isolated in M1 generation of S. splendens. Random amplified polymorphic DNA (RAPD) analysis demonstrated that DNA variations existed among the wild-type, fresh and dark red flower shoots of the chimera. The dark red flower shoots of the chimera were conserved and cultivated at a large-scale through micropropagation. MS supplemented with 2.0 mg/L BA and 0.3 mg/L NAA was the optimal medium in which the maximum proliferation ratio (5.2-fold) and rooting rate (88%) were achieved after 6 weeks. Our findings provide an important method to improve the ornamental quality of S. splendens. (c) 2009 Elsevier B.V. All rights reserved. |
WOS关键词 | BEAM IRRADIATION ; CELL-LAYERS ; CHRYSANTHEMUM ; FLORICAULA ; CULTURES ; PETALS ; SHAPE |
资助项目 | Program for New Century Excellent Talents in University (NCET) ; Gansu Province Program for Research and Exploitation of Agriculture Biotechnology[GNSW-2006-16] |
WOS研究方向 | Agriculture |
语种 | 英语 |
WOS记录号 | WOS:000267007100012 |
出版者 | ELSEVIER SCIENCE BV |
资助机构 | Program for New Century Excellent Talents in University (NCET) ; Gansu Province Program for Research and Exploitation of Agriculture Biotechnology |
公开日期 | 2010-10-29 |
源URL | [http://ir.imp.cas.cn/handle/113462/5285] ![]() |
专题 | 近代物理研究所_近代物理研究所知识存储(2010之前) |
通讯作者 | Hou, S. W. |
作者单位 | 1.Lanzhou Univ, Sch Life Sci, Key Lab Arid & Grassland Ecol, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China |
推荐引用方式 GB/T 7714 | Wu, D. L.,Hou, S. W.,Qian, P. P.,et al. Flower color chimera and abnormal leaf mutants induced by C-12(6+) heavy ions in Salvia splendens Ker-Gawl.[J]. SCIENTIA HORTICULTURAE,2009,121(4):462-467. |
APA | Wu, D. L.,Hou, S. W.,Qian, P. P.,Sun, L. D.,Zhang, Y. C.,&Li, W. J..(2009).Flower color chimera and abnormal leaf mutants induced by C-12(6+) heavy ions in Salvia splendens Ker-Gawl..SCIENTIA HORTICULTURAE,121(4),462-467. |
MLA | Wu, D. L.,et al."Flower color chimera and abnormal leaf mutants induced by C-12(6+) heavy ions in Salvia splendens Ker-Gawl.".SCIENTIA HORTICULTURAE 121.4(2009):462-467. |
入库方式: OAI收割
来源:近代物理研究所
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