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A quick isolation method for mutants with high lipid yield in oleaginous yeast

文献类型：期刊论文

作者Li, Renmin; Lu, Dong; Ma, Shuang; Wang, Jufang; Yan, Yaping; Li, Wenjian
刊名WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
出版日期2009-05-01
卷号25期号:5页码:921-925
关键词Oleaginous yeast Mutant Cerulenin Sulfo-phospho-vanillin reaction
ISSN号0959-3993
DOI10.1007/s11274-009-9960-2
英文摘要A novel method has been developed to easily isolate the mutants with high lipid yield after irradiating oleaginous yeast cells with carbon ions of energy of 80 MeV/u. Pre-selection of the mutants after ion irradiation was performed with culture medium in which the concentration of cerulenin, a potent inhibitor of fatty acid synthetase, was at 8.96 mu mol/l. Afterwards, lipid concentration in the fermentation broth of the pre-selected colonies was estimated by the sulfo-phospho-vanillin reaction instead of the conventional methanol-chloroform extraction. Two mutants with high lipid yield have been successfully selected out by the combined method. This easy and simple method is much less time-consuming but very efficient in the mutant isolation, and it has demonstrated great potential on mutation breeding in oleaginous microorganism.
WOS关键词FATTY-ACID-COMPOSITION ; SINGLE-CELL OIL ; BIOSYNTHESIS ; EXTRACTION ; CULTURE
WOS研究方向Biotechnology & Applied Microbiology
语种英语
WOS记录号WOS:000264970200025
出版者SPRINGER
公开日期2010-10-29
源URL[http://ir.imp.cas.cn/handle/113462/5347]  
专题近代物理研究所_近代物理研究所知识存储（2010之前）
近代物理研究所_生物物理研究室
通讯作者Wang, Jufang
作者单位Chinese Acad Sci, Radiobiol Lab, Inst Modern Phys, Lanzhou 730000, Peoples R China
推荐引用方式
GB/T 7714		 Li, Renmin,Lu, Dong,Ma, Shuang,et al. A quick isolation method for mutants with high lipid yield in oleaginous yeast[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2009,25(5):921-925.
APA Li, Renmin,Lu, Dong,Ma, Shuang,Wang, Jufang,Yan, Yaping,&Li, Wenjian.(2009).A quick isolation method for mutants with high lipid yield in oleaginous yeast.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,25(5),921-925.
MLA Li, Renmin,et al."A quick isolation method for mutants with high lipid yield in oleaginous yeast".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 25.5(2009):921-925.

入库方式： OAI收割

来源：近代物理研究所

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